The purpose of the current study was to report a simple and practical method to prepare high molecular weight (mw) DNA ladders. The method involves 1,000-4,000-base pairs (bp) DNA fragments being amplified by polymerase chain reaction (PCR), using λ DNA as a template. The constructed plasmids are digested by restriction endonucleases to produce 5-...

The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the existence of two groups. Group 1 shows a 195 bp repeated unit (Hinf I) and high ...

Repetitive DNA sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of such sequences provide an insight into the organization of the genome of interest. Here, we report the isolation and molecular characterization of a novel repetitive DNA sequence from the genome of Citrus limon. Digestion of C. limon DNA with MboI produced a prominen...

aim : the aim of this study was to investigate the prevalence of gbv-c among iranian hbv positive patients using pcr-rflp technique. background : gbv-c was a member of flaviviridae family and recently propose to classify as members of a fourth genus in this family, named pegivirus  and suggest that at least one quarter of the world's population has been infected with this virus. gbv-c can be tr...

a graph g = (v,e) with p vertices and q edges is said to be a total mean cordial graph if there exists a function f : v (g) → {0, 1, 2} such that f(xy) = [(f(x)+f(y))/2] where x, y ∈ v (g), xy ∈ e(g), and the total number of 0, 1 and 2 are balanced. that is |evf (i) − evf (j)| ≤ 1, i, j ∈ {0, 1, 2} where evf (x) denotes the total number of vertices and edges labeled with x (x = 0, 1, 2). in thi...

background and objectives: dna ladder contains dna fragments of different length but with known size, used to determine the size of unknown dna molecules. different dna ladders are available for expected dna length. conserved sequences were selected for design of primers to generate dna fragments of known specific size. materials and methods: in this study, we describe a method by which dna lad...

Corticotropin-releasing factor-binding protein (CRF-BP) is known to regulate the bioavailability of CRF and may also play a role in stress behaviours. CRF-BP has been localized in the pituitary as well as central nervous system (CNS) limbic and cortical areas, including the amygdala. The signal transduction pathways which regulate amygdalar CRF-BP are not well understood. In this report, we hav...