Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits. A combination 6-benzylaminopurine (BAP) and thidiazuron (TDZ) plant growth regulators was applied evaluate shoot capacity whereas α-naphthalene acetic acid (NAA) with (BAP), 2, 4-dichlorophenoxyacetic (2, 4-D) were tested optimize root induction two peanut culti...

dog rose (rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. it can be propagated under in vitro conditions. after removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on murashige and skoog medium (ms ...

tissue culture methods provide tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, in vitro propagation of diaphanoptera khorasanica rech.f., a rare and threatened plant species with limited distribution range and population was investigated. this species has a potential as an ornamental plant. single node explants wer...

La finalidad de este estudio fue evaluar la capacidad regeneración yemas adventicias en explantes segmentos hojas y entrenudos provenientes plantas in vitro. Para ello, fueron germinadas semillas Balfourodendron riedelianum condiciones vitro, luego un mes los cultivados medio Murashige Skoog suplementado con combinaciones ANA (0.01 mg.L-1), BAP (0.1 mg.L-1) TDZ (0.1, 0.01 mg.L-1). Los mejores r...

The use of in vitro culture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis (L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plan...

The effects o f thidiazuron (TDZ) on adventitious shoot bud formation from leaf and peti­ ole explants excised from in-vitro-grown plantlets o f a begonia were tested by a two-step procedure: a short exposure (5 to 120 min) to 40 pM TDZ in liquid MS medium followed by culture on agar-solidified MS medium without growth regulators. Exposure of explains for five min to TDZ induced bud formation, ...

Grass pea (Lathyrus sativus L.) is highly nutritious and protein rich legume crop; however, presence of ODAP (β-NOxalyl-L-α, β-diaminopropionic acid) has restricted its use for human and animal consumption. There is need for accelerated breeding of low or zero ODAP cultivars to ensure safer and wider use of grass pea through conventional and biotechnological approaches. The study investigated s...

The gerbera market would benefit from an efficient and simple protocol for high rate regeneration for propagation and genetic engineering. With these objectives, this investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii ‘Royal Soft Pink’. Murashige and Skoog (1962) (MS) medium was supplemented with 0.1 mg L-1 indole-3-acetic acid...