Proteases are important industrial enzymes used in different areas of industry, mainly detergent, food and leather industries. In this study, novel alkaline protease-producing bacterium was isolated from Geinarje hot spring and examined for maximum protease activity to be utilized in washing-powder. The isolated bacterium was cultured in mineral salt medium including 2% Skim Milk. Proteolytic a...

The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...

Syzygium aromaticum merupakan tanaman obat yang terkenal akan kegunaannya di dunia medis. Tanaman ini mengandung minyak esensial memiliki banyak bioaktivitas yakni eugenol. Penelitian bertujuan untuk mengetahui potensi senyawa eugenol dan turunannya sebagai inhibitor HIV-1 Protease (PR) kandidat antivirus HIV-1. Ligan digunakan dalam penelitian adalah eugenol, metil asetil isoeugenol. Hasil men...

Several nonpeptide small molecules were designed as potential inhibitors of HIV protease and their structures were constructed by computer-aided molecular modeling and docked iwo the active site of HIV protease. Models of the complexes of inhibitors and the HIV protease were refined using nonbonded and H-bonding terms. The refined energy of selected complexes showed that the designed inhib...