background and objectives: grape has high nutritional value and it is an economically important horticultural plant in the world. iran has seventh producer grape rank in the world. today tissue culture is one of technique which can be used in grape propagation and breeding. material and method: in order to optimization of callus induction and micropropagation of grape in vitro condition, this i...

In vitro plantlet regeneration has been obtained from 20-25days old seedling shoot tip segments of Cucumis melo (L.). We were examined using various phyto hormones individually and in combination on Murashige and Skoog (MS) semi solid medium supplemented with BAP (1.0-3.0 mg/L) Kn (1.0-3.0 mg/L),IAA (0.5 mg/L)+ BAP (1.0-3.0 mg/L) and IAA (0.5 mg/L)+Kn (1.0-3.0 mg/L) for multiple shoots prolifer...

An efficient method for in vitro micro propagation and genetic transformation of plants are crucial for both basic and applied research. Maize is one of the most important cereal crops around the world. Regeneration from immature embryo is hampered due to its unavailability round the year. On the contrary mature embryo especially tropical maize is recalcitrant toward tissue culture. Here we rep...

A rapid and efficient in vitro propagation protocol of Acorus calamus L. an important endangered medicinal plant has been developed. Naturally grown rhizome segments were used as explants for initial culture. Surface sterilized rhizome segments were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations (0.5 mg l 3.0 mg l ) of cytokinins (BAP, Kn) and auxins (IAA, ...

Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/l) with NAA (0.2-1.0 mg/l) and BAP (1.0-3.0 mg/l) with Kn. (0.2-1.0 mg/l) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/l). Subculture on the same medium resulted in rapid shoot multiplication at an a...

abstract in vitro leaf explants of gf677 hybrid rootstock and rabi cultivar were placed on mql medium supplemented with bap at 0, 1, 2, 3 and 4 mg/l and naa at 0, 0.15 and 0.3 mg/l concentrations. the results revealed that bap, naa, genotype and their interactions had significant effect on both percentage and mean number of the regenerated shoots. for gf677, the highest percentage of shoot rege...

OBJECTIVE To evaluate the possible vascular effects of an environment carcinogen benzo(a)pyrene (BaP). METHODS The cytotoxicit of BaP and rat liver S9 (0.25 mg/mL)-activated BaP were examined by MTT assay. Thoracic aortic rings were dissected from Sprague-Dawley rats. Contraction of aortic rings was induced by 60 mmol/L KCl or 10(-6) mol/L phenylephrine (PE) in an ex-vivo perfusion system aft...