Ca2+ binding is essential for the biological functions of calmodulin (CaM) as a trigger/sensor protein to regulate many biological processes in the Ca2+ -signaling cascade. A challenge in understanding the mechanism of Ca2+ signaling is to obtain site-specific information about the Ca2+ binding properties of individual Ca2+ -binding sites of EF-hand proteins, especially for CaM. In this paper, ...

Glu is highly conserved as the first amino acid of E-helix of the EF-hand protein. In this paper, Glu 101, the first amino acid of E-helix of the third EF-hand motif in Euplotes octocarinatus centrin (EoCen) was mutated to be Lys by the method of site direct mutation. Tb3+ and TNS were used as fluorescence probes in the study of the effect of this mutation to the metal binding characteristic of...

Uniform monodisperse LuPO4:Eu3+/Tb3+ hollow microspheres with diameters of about 2.4 µm have been successfully synthesized by the combination of a facile homogeneous precipitation approach, an ion-exchange process and a calcination process. The possible formation mechanism for the hollow microspheres was presented. Furthermore, the luminescence properties revealed that the LuPO4:Eu3+ and LuPO4:...

Quality-Assuring Scheduling (QAS) has been invented to provide statistical guarantees to real-time applications with variable execution times scheduled in a reservation-based fixed-priority system. The admission control is based on a probabilistic model to ensure that a requested percentage of jobs of a periodic application is successfully executed over a longer time span even in permanent over...

Previously we have shown that the fluorescence of the dihydropyridine calcium antagonist felodipine provides an accurate means of monitoring the formation of an allosterically potentiated conformer of calmodulin (Mills, J. S., and Johnson, J. D., (1985) Biochemistry 24, 4897-4903). Characteristic of this conformer is the abolition of cooperativity among the two felodipine-binding sites on calmo...

CONTEXT Thera-Band (TB) exercises are commonly utilized in ankle rehabilitation, but previous studies have shown inconsistent results. OBJECTIVE To compare two TB protocols among healthy subjects in improving postural control. DESIGN Mixed model design. SETTING Research laboratory. PARTICIPANTS Thirty healthy subjects divided into a control (CON), three times/week (TB3) or five times/we...

In the title compound, [Tb(C2H3O2)2(C11H10N4)(H2O)2]NO3·H2O, the Tb3+ ion is nine-coordinated in a distorted tricapped trigonal-prismatic geometry by the three N atoms of the tridentate 1-(pyridin-2-yl-methyl-idene)-2-(pyridin-2-yl)hydrazine ligand, four carboxyl-ate O atoms of two chelating acetate groups and two O atoms of the coordinating water mol-ecules. The organic hydrazine ligand is dis...

A new sensitive, simple and rapid method for determination of methotrexate (MTX) was developed based on quenching effects of MTX on the fluorescence intensity of Tb3+-1,10-phenanthroline complex. The fluorescence intensity was measured with excitation wavelength of 300 nm and emission wavelength of 545 nm and the quenched fluorescence intensity is proportional to the concentration of MTX in Tris...