quinolones are a large and widely consumed class of synthetic drugs. expanded-spectrum quinolones, like ciprofloxacin are highly effective against gram-negative bacteria, especially escherichia coli. in e. coli the major target for quinolones is dna gyrase. this enzyme is composed of two subunits, gyra and gyrb encoding by gyra and gyrb, respectively. mutations in either of these genes cause qu...

Quinolones are a large and widely consumed class of synthetic drugs. Expanded-spectrum quinolones, like ciprofloxacin are highly effective against Gram-negative bacteria, especially Escherichia coli. In E. coli the major target for quinolones is DNA gyrase. This enzyme is composed of two subunits, GyrA and GyrB encoding by gyrA and gyrB, respectively. Mutations in either of these genes cause qu...

BACKGROUND The detection of mutations in the gyrA and gyrB genes in the Mycobacterium tuberculosis genome that have been demonstrated to confer phenotypic resistance to fluoroquinolones is the most promising technology for rapid diagnosis of fluoroquinolone resistance. METHODS In order to characterize the diversity and frequency of gyrA and gyrB mutations and to describe the global distributi...

Ftorhinolony — osnovnaya gruppa preparatov, primenyaemyh dlya lecheniya tuberkuleza s mnozhestvennoj lekarstvennoj ustojchivost'yu (MLU-TB). Cel'yu issledovaniya bylo ocenit' raznoobrazie mutacij v gene gyrA, a takzhe ustanovit' associaciyu gyrA fenotipicheskoj k levofloksacinu i obshchim profilem ustojchivosti vozbuditelya. Issledovanie provedeno na diagnosticheskom materiale ot 2836 bol'nyh t...

AIM To analyse mutations in the gyrA and parC genes leading to possible increase in ciprofloxacin resistance (high MIC values for ciprofloxacin) in clinical isolates of Neisseria gonorrhoeae in Delhi, India. METHOD MIC of ciprofloxacin for 63 clinical isolates of N gonorrhoeae were examined by the Etest method. Subsequently, gyrA and parC genes of these isolates were amplified and sequenced f...

conclusions this study demonstrated the specificity and sensitivity of the pcr-sscp method for finding mutations in the gyra gene. due to the sensitivity of most isolates to ciprofloxacin, this antibiotic should be considered an appropriate drug for the treatment of related diseases. background during the past several years, nontuberculous mycobacteria (ntm) have been reported as some of the mo...

DNA gyrase catalyses the adenosine triphosphate-dependent introduction of negative supercoils into DNA. The enzyme binds a DNA-segment at the so-called DNA-gate and cleaves both DNA strands. DNA extending from the DNA-gate is bound at the perimeter of the cylindrical C-terminal domains (CTDs) of the GyrA subunit. The CTDs are believed to contribute to the wrapping of DNA around gyrase in a posi...