هدف: از چالش­های عمده بشریت افزایش افسردگی و اختلال عملکرد جنسی ناشی داروهای ضدافسردگی است. با توجه به نقش سلول­های سرتولی در اسپرماتوژنز، پژوهش حاضر، اثر داروی دولوکستین را بر زنده­مانی، آپوپتوزیس بیان ژن­های Bax و­ (Connexin 43) Cx43 بررسی کرده مواد روش‌‌ها: TM4 محیط DMEM/F12 حاوی %5/2 FBS، 5% سرم اسب %1 پنی سیلین-استرپتومایسین کشت شدند. دوزهای 30،60، 15، 5/7، 75/3 میکرو­گرم/ میلی­لیتر زمان­ه...

Casein genes directly control milk protein of animals. CSN1S1 (αS1-Casein) and CSN2 (β-Casein) influence on fractions. Genetic polymorphisms gene at g.12164G>A locus g.8913C>A were identified by PCR-RFLP technique. Animal samples pure dairy goats providing PE (5 hds.), Saanen (8 hds.) their crosses Sapera (50% Saanen, 50% PE) (51 SaanPE (75% 25% (3 from IRIAP goat station. Allele frequenc...

The aim of this work was to evaluate the variability of the as1-casein locus in the endangered Girgentana dairy goat breed in order to define genetic improvement and a conservation program for this breed. The study was performed on 200 dairy goats by means of different PCR protocols. Themost frequent alleles were A (0.590) and F (0.290) followed by B (0.065) and N (0.047).CSN1S1 E allele was id...

Genetic variations in the four casein genes CSN1S1 , CSN2 CSN1S2 and CSN3 have obtained substantial attention since they affect milk protein yield, composition, cheese processing properties, digestibility as well tolerance human nutrition. Furthermore, variants are used for breed characterization, biodiversity, phylogenetic studies. The current study aimed at identification of five domestic goa...

The COP9 signalosome (CSN) is involved in multiple developmental processes. It interacts with SCF ubiquitin ligases and deconjugates Nedd8/Rub1 from cullins (deneddylation). CSN is highly expressed in Arabidopsis floral tissues. To investigate the role of CSN in flower development, we examined the expression pattern of CSN in developing flowers. We report here that two csn1 partially deficient ...