This study was accomplished to detect variation generated through eposure of fenugreek (trigonella foenum-graecum L.) seeds seven gamma irradiation doses (0,25,50,100,150,300 and 400) GY by using twenty RAPDs primers as molecular marker ,results indicated that OPA-14 ,OPC-19 OPH-01success in giving five treatments a unique fingerprint. Molecular size amplicon ranged between 2913 bp produced pri...

Random Amplified Polymorphic DNAs (RAPDs) data were analyzed from eighteen taxa of Cupressus from the eastern hemisphere. The following fourteen taxa were distinct in their RAPDs: Cupressus assamica (India), C. atlantica (Morocco), C. austrotibetica (Tibet), C. cashmeriana (Bhutan), C. chengiana (Sichuan), C. darjeelingensis (India), C. duclouxiana (Yunnan), C. dupreziana (Algeria), C. funebris...

Population phylogeographic studies are generally based solely on mtDNA without corroboration, from an independent segregating unit (i.e., nuclear genes), that the mtDNA gene tree represents the organismal phylogeny. This paper attempts to evaluate the utility of microsatellites for this process by use of the Western Canary Island lacertid (Gallotia galloti) as a model. The geological times of i...

Random amplified polymorphic DNAs (RAPDs) data were analyzed from all the taxa of Cupressus from the western hemisphere. Populations of Cupressus from Arizona and Texas, USA, were found to cluster in the two groups delimited by Wolf (1948): C. arizonica and C. glabra. These data suggest that these taxa might be better recognized at the specific level as per Wolf (1948), rather than at the varie...

1 Introduction The first step of RAPD fingerprinting is the preparation of the target DNA template. Intuitively, minimal DNA template preparation should be necessary for RAPDs since, theoretically, PCR may amplify a single DNA molecule. It seems one would simply homogenize tissue and allow the PCR to "find" and amplify the target DNA. Indeed, many rapid DNA isolation methods designed for use wi...

Borderea chouardii is a relictual and dioecious, strictly sexually reproducing, long-living geophyte of the Dioscoreaceae family. Previous biological and demographic studies have indicated the existence of a uniformly distributed panmictic population of this taxon at the southernmost Spanish pre-Pyrenean mountain ranges where it occurs in rather inaccessible crevices of a single limestone cliff...