The aim of this work was to compare use of an o-phthaldialdehyde (OPA) colorimetric assay (OPA-C), which responds to both free AA and peptides, with an OPA fluorimetric assay (OPA-F), which is insensitive to peptides, to quantify rates of ruminal protein degradation in the inhibitor in vitro system using Michaelis-Menten saturation kinetics. Four protein concentrates (expeller-extracted soybean...

BACKGROUND The behavioural and socio-cultural processes underlying the association between socio-economic position (SEP) and body mass index (BMI) remain unclear. Occupational physical activity (OPA) is one plausible explanatory variable that has not been previously considered. OBJECTIVES 1) To examine the association between OPA and BMI, and 2) to examine whether OPA mediates the SEP-BMI ass...

Early diagnosis of Neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. We report the development of a specific and sensitive TaqMan assay for the detection of N. gonorrhoeae in clinical samples. The target sequence is a 76-bp fragment of the 5' untranslated region of the opa genes that encode opacity proteins. A panel of 448 well-defined N. gonorrhoeae...

Escherichia coli O157:H7, an emerging cause of food-borne disease, has now been reported from several countries worlwide. Cattle and sheep are known as a principle reservoir of this agent. It is also prevalent in gastrointestinal tract of other animals like pigs, dogs, cats and birds. This agent can be transmitted to humans by food and water and even person to person contact. The random amplifi...

The diagnostic value of various signs and symptoms (clinical markers) in predicting oropharyngeal aspiration (OPA) or swallowing dysfunction has not been established in children. The present retrospective study was undertaken to: 1) identify specific clinical markers associated with radiographic evidence of OPA, isolated laryngeal penetration (ILP) and post-swallow residue (PSR); 2) determine t...

BACKGROUND Antibiotic resistance and decreased susceptibility to disinfectants are not usually associated in microorganisms, but we have found an exception to this rule: P. aeruginosa versus orthophthalaldehyde (OPA). METHODS Bactericidal effect of OPA was measured at 10 minutes on endodoncy files contaminated with an ATCC strain (control) or 206 strains of P. aeruginosa recently isolated fro...

Neuroimaging studies have identified multiple scene-selective regions in human cortex, but the precise role each region plays in scene processing is not yet clear. It was recently hypothesized that two regions, the occipital place area (OPA) and the retrosplenial complex (RSC), play a direct role in navigation, while a third region, the parahippocampal place area (PPA), does not. Some evidence ...

OBJECTIVE Leisure-time physical activity (LTPA) has considerable benefits for cardiovascular health and longevity, while occupational physical activity (OPA) is associated with an elevated cardiovascular risk. This "health paradox" may be explained by different effects on the autonomic nervous system from OPA and LTPA. Thus, we aimed to investigate whether objectively measured OPA and LTPA are ...