We have tested the characteristics of the method of Iwata and Nishikaze (Clin Chem 25: 1317, 1979). The linearity, sensitivity, and precision are satisfactory and the reactivity of benzethonium chloride with various proteins (albumin, immunoglobulins) is the same. The method has been compared with Meulemans's technique (Clin Chim Acta 5: 757, 1960), routinely used in our laboratories, by analys...

TGF/Smad3 promotes renal fibrosis, but the mechanisms that regulate profibrotic genes remain unclear. We hypothesized that miR-192, a microRNA expressed in the kidney may mediate renal fibrosis in a Smad3-dependent manner. Microarray and real-time PCR demonstrated a tight association between upregulation of miR-192 in the fibrotic kidney and activation of TGF/Smad signaling. Deletion of Smad7 p...

1. Van Biesen W, Lameire N, Vanholder R. Why less success of the peritoneal dialysis programmes in Europe? Nephrol Dial Transplant 2008; 23: 1478–1481 2. Mehrotra R, March D, Vonesh E et al. Patient education and access of ESRD patients to renal replacement therapies beyond in-center hemodialysis. Kidney Int 2005; 68: 378–390 3. Khawar O, Kalantar-Zadeh K, Lo WK et al. Is the declining use of l...

The EC4 European syllabus for post-graduate training in clinical chemistry and laboratory medicine: Version 4 2012. Wieringa G, Zerah S, Jansen R, Simundic AM, Queralto J, Solnica B, Gruson D, Tomberg K, Riittinen L, Baum H, Brochet JP, Buhagiar G, Charilaou C, Grigore C, Johnsen AH, Kappelmayer J, Majkic-Singh N, Nubile G, O’ Mullane J, Opp M, Pupure S, Racek J, Reguengo H, Rizos D, Rogic D, S...

By using a benzethonium chloride concentration 12-fold that described originally (Clin Chem 1979;25:1317-9), we developed a reliable method suitable for routine measurement of total protein in cerebrospinal fluid. Only 10 microL of sample is required. Reactivity to immunoglobulin G (IgG) and to albumin (Alb) is similar, as is necessary for specimens that can have very varied IgG/Alb ratios. The...

زمینه و هدف: هدف از پژوهش حاضر بررسی تأثیر تمرینات ترکیبی بر سطح استراحتی پنتراکسین3 (PTX3) سرم آمیلوئید A(SAA) پلاسمای مردان مبتلا به دیابت نوع دو بود تا بدین‌وسیله، ورزش کاهش عوامل التهابی فوق در افراد دیابتی سنجیده شود.مواد روش‌ها: ۲۰ مرد غیرورزشکار دو، تصادفی گروه کنترل تمرین توزیع شدند. مدت شش هفته شامل 25-35 دقیقه هوازی با شدت 55-75 درصد ضربان قلب بیشینه پنج حرکت مقاومتی دربرگیرندة عضلات ...