materials and methods in the present study, a potent nk-producing strain was isolated from cow milk and identified. to enhance the yield of nk, effect of various parameters such as ph, temperature, carbon source, nitrogen source and inoculum size were optimized. strain improvement of p. aeruginosa cmss was done by random uv-mutagenesis. nattokinase was partially purified and the activity was de...

Exosomes are released by various cells, including natural killer (NK) cells and transport signaling molecules for the intercellular communication. Hepatocellular carcinoma (HCC), also known as primary liver cancer, is often inoperable difficult to accurate diagnosis. Notably, prognosis underlying mechanisms of HCC not fully understood. Exosomes-derived NK (NK-exos) express unique cytotoxic prot...

The aim of this paper is a) if Σak2 < ∞ then Σak rk(x) is in BMO that{rk(x)} is Rademacher system. b) P1k=1 ak!nk (x) 2 BMO; 2k nk < 2k+1that f!n(x)g is Walsh system. c) If jakj < 1k then P1k=1 ak!k(x) 2 BMO.

conclusions our results suggested that cb-nk cells progenitors could proliferate and differentiate on feeder layer of amnion membrane mscs in combination with specific cytokines to produce nk cells for immunotherapy. results combination of cytokines and msc layer yielded significant fold expansion in comparison with cytokines without feeder conditions (day 7: 5.2 ± 1.12 and 2 ± 0.78, respective...

Background: HLA-G is a nonclassical HLA class I molecule which, when elevated in tumor cells, is one of the main factors involved in tumor evasion of immune responses including NK and T cells. Objective: To evaluate the effect of HLA-G downregulation on NK cell cytotoxicity in tumor cell lines. Methods: The expression level of HLA-G was measured by real-time PCR and flowcytometry after transfec...

The effect of natural killer (NK) cells and lymphokine activated killer ( LAK) cells was studied on a group of human melanoma cell lines. Peripheral blood from healthy volunteers was utilized as a fresh source of natural killer cells and rhI L-2 for producing LAK cells. The cytotoxicity of effector cells was quantified using a 4 hour SI determining the density of antigen expression on tumor...

Background: Recurrent spontaneous abortion (RSA) and in vitro fertilization (IVF) failure with unknown causes are the controversial issues that are probably related to the immune system. Objective: To compare circulating NK cells expressing activation and inhibition surface markers between patients with RSA and IVF failure with those of healthy multiparous and successful IVF control women, resp...

Abstract Natural Killer (NK) cell-mediated cytotoxicity is a critical mechanism of the immune system to respond against harmful stimuli. The development emerging cell therapies, such as CAR-NK cells, requires deeper characterization cultured NK cells. Here we show phenotyping ex vivo and in vitro demonstrate how high quality reliable reagents from BioLegend ensure more depth cytotoxic analysis....

background: recurrent spontaneous abortion (rsa) and in vitro fertilization (ivf) failure with unknown causes are the controversial issues that are probably related to the immune system. objective: to compare circulating nk cells expressing activation and inhibition surface markers between patients with rsa and ivf failure with those of healthy multiparous and successful ivf control women, resp...