DEAE-Dextran, Dunaliella, Membrane Permeability, Photosynthesis, Polycations As a prerequisite for studies o f the intracellular distribution o f enzymes o f the glycerol cycle in Dunaliella parva, the effect o f the polycation DEAE-dextran on the permeability o f membranes for various endogenous compounds and on photosynthesis was investigated. DEAE-dextran induces an increase o f the permeabi...

We carried out the purification of C-phycocyanin and allophycocyanin from Spirulina platensis taking advantage of the adsorption properties of the expanded beds. Initially, phycobiliproteins were released from the microalga cells by osmotic shock. Next, phycocyanins were recovered by applying the centrifuged cell suspension directly to the anion exchanger Streamline-DEAE using expanded bed colu...

Expanded bed adsorption chromatography (EBAC) is a single pass operation that has been used as primary capture step in various protein purifications. The most common problem in EBAC is often associated with successful formation of a stable fluidized bed during the absorption stage, which is critically dependent on parameters such as liquid velocity, bed height, particle (adsorbent) size and den...

The study included the extraction and purification of glucose oxidase enzyme from Penicillium chrysogenum, pure by precipitation with ammonium sulfate at a saturation rate 70% specific activity in this step reached (10.802) units/mg fold 1.276, was followed dialysis step, 13,593 48.184. Finally, ion exchange chromatography using DEAE-Cellulose column, 19.355 units/mg, 36.59, after which migrate...

Electroporation has proven to be a convenient method for transfection of many cell types. One drawback has been that it requires large amounts of DNA, typically 20 to 80 /tg per transfection (1). Here we report that adding DEAE-dextran to the electroporation media dramatically increases electroporation efficiency in mammalian cells, lowering the amount of DNA required for efficient transfection...

in this study, we present changes of peroxidase (e. c. 1.11.1. 7) activities in the root of brassica napus var. okapi during rosette stage until ripening of the fruits. peroxidase activity was determined seasonally over an eight month period. results showed that total peroxidase activity in the root was highest in mid june and the lowest activities were observed from time of winter dormancy unt...

History matching large reservoir models under a realistic geological continuity constraint remains an outstanding problem. We propose a new combined geostatistical and streamline-based method potentially capable of history matching large reservoir models, while accounting consistently for geological continuity as provided by a permeability histogram and variogram, and production data. While mos...

in this article, we study the new streamline diffusion finite element for treating the linear second order hyperbolic initial-boundary value problem. we prove a posteriori $ l^2(l^2)$ and error estimates for this method under minimal regularity hypothesis. test problem of an application of the wave equation in the laser is presented to verify the efficiency and accuracy of the method.