INTRODUCTION We aimed to design a real-time reverse-transcriptase-PCR (rRT-PCR), high-resolution melting (HRM) assay to detect the H275Y mutation that confers oseltamivir resistance in influenza A/H1N1 2009 viruses. FINDINGS A novel strategy of amplifying a single base pair, the relevant SNP at position 823 of the neuraminidase gene, was chosen to maintain specificity of the assay. Wildtype a...

Single nucleotide polymorphisms (SNPs) of VKORC (1173T/C, rs9934438) and CYP2C9 (1075A/C, rs1057910) are major contributory factors on the sensitivity of warfarin in Chinese. Analysis of the two genomic loci could help warfarin treatment individual from bleeding or thrombosis events. An assay with the advantages of simplicity, speed, high sensitivity and low cost for genotyping is calling for c...

High-resolution melting (HRM) shows great promise for high-throughput, rapid genotyping of individual polymorphic loci. We have developed HRM assays for genotyping single nucleotide polymorphisms (SNP) in several key genes that are involved in methyl metabolism and may directly or indirectly affect the methylation status of the DNA. The SNPs are in the 5,10-methylenetetrahydrofolate reductase (...

Thiopurine intolerance and treatment-related toxicity, such as fatal myelosuppression, is related to non-function genetic variants encoding thiopurine S-methyltransferase (TPMT) and Nudix hydrolase 15 (NUDT15). Genetic testing of the common variants NUDT15:NM_018283.2:c.415C>T (Arg139Cys, dbSNP rs116855232 T allele) and TPMT: NM_000367.4:c.719A>G (TPMT*3C, dbSNP rs1142345 G allele) in East Asia...

objectives: human cytomegalovirus (cmv) is a major life-threatening pathogen for hematopoietic stem cell transplant recipients. specific tests are used for the diagnosis and monitoring of cmv infection in transplant patients. this study evaluates the performance of pp65 antigenemia and qualitative pcr assays for monitoring cmv in such patients. methods: we analyzed 179 clinical samples from 41...

viruses are the most common causes of aseptic meningitis. early detection, treatment and management of viral meningitis are priority. this study aimed to evaluate common viral meningitis in children referred to taleghani pediatrics hospital in gorgan, south east of caspian sea, iran. in this descriptive study csf and blood samples were taken from 40 children with negative bacterial culture who ...