This study describes a PCR-based approach for the production of a rationally attenuated mutant of Yersinia pestis. Degenerate primers were used to amplify a fragment encoding 91.45% of the aroA gene of Y. pestis MP6 which was cloned into pUC18. The remainder of the gene was isolated by inverse PCR. The gene was sequenced and a restriction map was generated. The Y. pestis aroA gene had 75.9% ide...

Title of Document: FRACTURE BEHAVIOR AND THERMAL CONDUCTIVITY OF POLYCRYSTALLINE GRAPHENE Andrew Fox, Doctor of Philosophy, 2014 Directed By: Associate Professor Teng Li, Department of Mechanical Engineering This dissertation investigates the effect of grain boundaries (GBs) in polycrystalline graphene on the tensile fracture behavior and thermal conductivity of the graphene sheets. Current tec...

Pendrin (PDS) and sodium/iodide symporter (NIS) are transmembrane proteins that are located in numerous tissue types, particularly thyroid follicular epithelial cells, where they are entrusted with the regulation of iodine molecules. In the present study, we aimed to clarify changes in PDS and NIS protein expression, in the testicular tissue of prepubertal and <span la...

An extract from the marine alga Ulva lactuca was highly osmoprotective in salt-stressed cultures of Sinorhizobium meliloti 102F34. This beneficial activity was due to algal 3-dimethylsulfoniopropionate (DMSP), which was accumulated as a dominant compatible solute and strongly reduced the accumulation of endogenous osmolytes in stressed cells. Synthetic DMSP also acted as a powerful osmoprotecta...

The interaction of grain boundaries (GBs) with inherent defects and/or impurity elements in multicrystalline silicon plays a decisive role their electrical behavior. Strain, depending on the types GBs and defects, an important these systems. Herein, correlation between structural electronic properties Σ3{111} Si-GB presence interstitial oxygen impurities is studied from first-principles framewo...

Pseudomonas putida GB-1 is a Mn(II)-oxidizing bacterium that produces pyoverdine-type siderophores (PVDs), which facilitate the uptake of Fe(III) but also influence MnO2 formation. Recently, a non-ribosomal peptide synthetase mutant that does not synthesize PVD was described. Here we identified a gene encoding the PVDGB-1 (PVD produced by strain GB-1) uptake receptor (PputGB1_4082) of strain GB...

glycine betaine (gb) is one of the osmotic plant-protecting members that accumulate in response to abiotic stresses. an experiment was, therefore, conducted to test whether gb application at various concentrations (0, 10, 20 and 30 mm) through seed soaking or foliar spray methods would protect cucumber seedlings, subjected to chilling stress. at two true leave stages, after gb treatment, the se...