background: brucellosis is one of the endemic and a zoonotic disease of the iran. science the rapid diagnosis and quick treatment are very important, selection of a sensitive and specific test solving this problem is of a great importance. the main aim of this study is comparison of routine serologic test with standard elisa test. materials and methods: in this survey, 176 suspected patients wi...

background: until now, the comparison of the rk39 strip test (rkt) and direct ag-glutination test (dat) for detection of visceral leishmaniasis (vl) is exclusively based on either positive or negative qualification of the reaction outcome. objective: in this study, we compared the diagnostic performance of rkt and dat for vl both qualitatively and semi-quantitatively. methods: for comparison ba...

Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. Cultures of Corynebacterium vaginalis, N. meningitidis, N. catarrhalis, N. sic...

We report kinetic studies on the reaction of a latex agglutination immunoassay used to quantify phenytoin in serum. In this assay, polystyrene particles with a covalently attached analog of phenytoin react with an antiphenytoin monoclonal antibody to form light-scattering aggregates, with the rate of this reaction being decreased by addition of phenytoin from sample. In the absence of free (sam...

In order to demonstrate that cells of Candida spp. may show considerable nonspecific agglutination in latex agglutination tests, 150 clinical and reference isolates of 12 yeast species were systematically studied by applying various test parameters. In fact, 40 (26.7%) of these isolates revealed nonspecific results, significantly associated with the time allowed for agglutination.

Hemagglutination inhibition, enzyme-linked immunosorbent assay (ELISA), and passive latex agglutination were used to test 495 human serum samples for determination of rubella immunity. Overall agreements for immune status were as follows: hemagglutination inhibition versus ELISA, 94.7% (469 of 495); hemagglutination inhibition versus passive latex agglutination (1:10 dilution), 99.2% (491 of 49...