A total of 74 phenotypically identified presumptive motile Aeromonas isolates recovered from septicaemic freshwater ornamental fish in Sri Lanka were genetically characterized by sequencing of rpoD and gyrB genes. rpoD/gyrB phylogeny confirmed only 53 isolates as Aeromonas, among which A. veronii was the predominant species (79.2%), followed by A. hydrophila (7.5%), A. caviae (5.7%), A. jandaei...

Five rRNA operons (rrn) were found by hybridization in the genome of 'Brevibacterium lactofermentum' ATCC 13869 and Corynebacterium glutamicum ATCC 13032. 'B. lactofermentum' DSM 20412 differed from the other corynebacteria tested in showing six hybridizing BamHI bands. Two of the rrn operons (rrnD and rrnE) were located in a single cosmid. Sequencing of the rrnD operon showed that it contains ...

Escherichia coli contains seven rRNA operons, each consisting of the genes for three rRNAs (16S, 23S and 5S rRNA in this order) and one or two tRNA genes in the spacer between 16S and 23S rRNA genes and one or two tRNA genes in the 3' proximal region. All of these rRNA and tRNA genes are transcribed from two promoters, P1 and P2, into single large precursors that are afterward processed to indi...

BACKGROUND Clone libraries provide researchers with a powerful resource to study nucleic acid from diverse sources. Metagenomic clone libraries in particular have aided in studies of microbial biodiversity and function, and allowed the mining of novel enzymes. Libraries are often constructed by cloning large inserts into cosmid or fosmid vectors. Recently, there have been reports of GC bias in ...

A multi locus sequence analysis (MLSA approach) was studied on the Bacillus genus, or subtilis species complex for specific, including 08 strains from four (B. subtilis, B. pumilus, licheniformis and amyloliquefaciens) were provided by Biotechnology Center of Ho Chi Minh City. The research based sequences 16S rRNA genes, concatenation five protein-coding housekeeping genes: glpF, pta, purH, pyc...

While the amount of molecular data that can be routinely generated continues to increase, advances in the methods of analysis to handle this quantity of data have not kept pace. One area of research which could greatly benefit from new approaches is the design of specific probes and PCR primers. Here we generated spanning networks (1) for phytopathogenic Pseudomonas strains from seven species, ...