Phycobiliproteins are brightly-fluorescent light-harvesting pigments for photosynthesis in cyanobacteria and red algae. They are also of interest as fluorescent biomarkers, but their heterologous generation in vivo has previously required multiple transformations. We report here a modular approach that requires only two DNA segments. The first codes for the apo-protein. The second codes for fus...

The λ-Red recombination system is a popular method for gene editing. However, its applications are limited due to restricted electroporation of DNA fragments. Here, we present an electroporation-free λ-Red recombination method in which target DNA fragments are excised by I-CreI endonuclease in vivo from the landing pad plasmid. Subsequently, the I-SceI endonuclease-cutting chromosome and DNA do...

Kengo Matsumoto has introduced λ-graph systems and strong shift equivalence of λ-graph systems [Doc.Math.4 (1999), 285-340]. We associate to a subshift of a subshift a λ-graph system. The strong shift equivalence class of the associated λ-graph system is an invariant of subsystem equivalence. Wolfgang Krieger and Kengo Matsumoto have introduced the λ-entropy of a λ-graph system and have shown i...

............................................................................................................................ vi LIST OF TABLES .................................................................................................................... x LIST OF FIGURES ................................................................................................................. xi LI...

Transonic planar flows around a circular cylinder are investigated numerically for laminar and turbulent flow conditions with Reynolds numbers of 50≤ReD≤300 8890≤ReD≤ 80,000 free stream Mach in the range 0.2≤Ma∞≤2. A commercially available CFD tool is used validated this purpose. The results show that phenomena occurring can be grouped into eight regimes. Compared to incompressible regimes, sev...

In this paper we present an algorithm for pricing barrier options in one-dimensional Markov models. The approach rests on the construction of an approximating continuous-time Markov chain that closely follows the dynamics of the given Markov model. We illustrate the method by implementing it for a range of models, including a local Lévy process and a local volatility jump-diffusion. We also pro...