The monoglyceride pathway for the synthesis of triglycerides has been investigated employing subcellular fractions and whole cell preparations of white and brown adipose tissue. Conclusive evidence has been obtained for the monoglyceride pathway in these tissues by employing the 2-monoether analogue of 2-monoolein as the substrate. The monoglyceride and alpha-glycerophosphate pathways were prim...

Acyl-CoA:glycerol-3-phosphate acyltransferase (GPAT) and acyl-CoA: 1-acyl-glycerol-3-phosphate acyltransferase (AGPAT) are involved in the de novo synthesis of triacylglycerol (TAG) and glycerophospholipids. Many enzymes belonging to the GPAT/AGPAT family have recently been identified and their physiological or pathophysiological roles have been proposed. The roles of GPAT/AGPAT in the synthesi...

Members of the haloacid dehalogenase (HAD) superfamily are emerging as an important group of enzymes by virtue of their role in diverse chemical reactions. In different Plasmodium species their number varies from 16 to 21. One of the HAD superfamily members, PVX_123945, a hypothetical protein from Plasmodium vivax, was selected for examining its substrate specificity. Based on distant homology ...

BLANCHAER, M. C. Respiration of mitochondria of red and white skeletal muscle. Am. J. Physiol. 206(5) :1o15-1020. I g64.-Mitochondria of guinea pig red and white skeletal muscle were isolated in a medium containing 0.25 M sucrose, I mM ethylenediamine tetraacetate, and I y0 fraction V bovine albumin. Oxidative phosphorylation with pyruvate-malate as substrate was tightly coupled in both types o...

For the investigation of the minimal structural requirements for cytokine induction, Staphylococcus aureus lipoteichoic acid derivatives with two, three, four, and five glycerophosphate backbone moieties, carrying each a d-alanyl residue, were needed. Based on two different glycerophosphate building blocks and 6b-O-phosphitylated gentiobiosyl diacylglycerol the desired target molecules (compoun...