The aim of this study was to investigate the effect of chlorfenvinphos administered at a single dose of 0.02 LD50 on the activity of serum of liver damage indicatory enzymes, such as β-glucoronidase (BGR), acid phosphatase (AcP) and alanine and aspartate aminotransferases (ALT and AST), as well as on the activities of superoxide dismutase (SOD) and catalase (CAT) in erythrocytes and liver. The ...

Abstract Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution the river. The objective this study was assess suitability a novel, simple and high throughput method developed our laboratory enumerate E. RW samples. based on use synthetic substrate specific for β-d-glucuronidase (GUS) produced by coli. GUS activities were monitored at eight se...

Abbreviations: ACL5, ACAULIS5; ATHB8, ARABIDOPSIS THALIANA HOMEOBOX8; bHLH, basic helix-loop-helix; 2,4-D IOE, isooctyl ester of 2,4-dichlorophenoxyacetic acid; EMS, ethyl methanesulfonate; GUS, β-glucuronidase; LHW, LONESOME HIGHWAY; mORF, main open-reading frame; MP, MONOPTEROS; PAO, POLYAMINE OXIDASE; SAC, SUPPRESSOR-OF-ACL5; SACL, SAC-LIKE; SAMDC, S-adenosylmethionine decarboxylase; TMO5, T...

Abbreviations: attE: attacin E gene ELISA: Enzyme Linked Immunosorbent Assay gusA: β-glucuronidase gene GUS: glucuronidase nos: nopaline synthase gene nptII: neomycin phosphotransferase II gene NPTII: neomycin phosphotransferase II protein PCR: polymerase chain reaction PTGS: post-transcriptional gene silencing RNA: ribonucleic acid RT-PCR: reverse transcription PCR T-DNA: transfer DNA TGS: tra...

In many eukaryotes, spliceosomal introns are able to influence the level and site of gene expression. The mechanism of this Intron Mediated Enhancement (IME) has not yet been elucidated, but regulation of gene expression is likely to occur at several steps during and after transcription. Different introns have different intrinsic enhancing properties, but the determinants of these differences r...

The Rhodobacter sphaeroides system has been used to express membrane proteins. However, its low yield has substantially limited its application. In order to promote the protein expression capability of this system, the pucC gene, which plays a crucial role in assembling the R. sphaeroides light-harvesting 2 complex (LH2), was overexpressed. To build a pucC overexpression strain, a pucC overexpr...

To survive, the entomopathogenic fungus Beauveria bassiana, which shows promise as a biocontrol agent for a variety of pests, including agricultural and forestry pests and vectors of human pathogens, must tailor gene expression to the particular pH of its environment. The pH response transcription factor gene BbPacC and its flanking sequence were cloned from this fungus. Quantitative reverse tr...

While somatostatin receptors (sst), through somatostatin-radiolabeled analogs, are used, mainly in second line, in the diagnosis and treatment of pheochromocytomas (PCC) and paragangliomas (PGL), the clinical significance of dopamine receptor subtype 2 (D₂) in PCC/PGL is unknown. Indeed, radiolabeled dopamine (DA) analogs such as fluorine 18 ((18)F)-DA, used for positron emission tomography in ...

Agrobacterium-mediated genetic transformation was explored for inducing insect resistance in mat rush (Juncus effusus L.). Firstly, the callus regeneration system was established, and secondly the Agrobacterium tumefaciens strain EHA105 with plasmid pKUB carrying cryIA(b), hph and gus genes, coding for hygromycin phosphotransferase and β-glucuronidase, respectively, was used for transformation....

The expression of DWARF4 (DWF4), which encodes a C-22 hydroxylase, is crucial for brassinosteroid (BR) biosynthesis and for the feedback control of endogenous BR levels. To advance our knowledge of BRs, we examined the effects of different plant hormones on DWF4 transcription in Arabidopsis thaliana. Semi-quantitative reverse-transcriptase PCR showed that the amount of the DWF4 mRNA precursor e...