Cow's milk protein allergy (CMPA) represents one of the leading causes food in infants and young children. The immune reaction mainly due to casein β-lactoglobulin protein. Many systems are affected specially GIT,respiratory system skin.

Mammalian milk is a complex fluid mixture of various proteins, minerals, and lipids, which play an important role in providing nutrition and immunity to the newborn. Casein proteins, which form about 80% of the bovine milk proteins, form large colloidal particles with calcium phosphate to form casein micelles, which for many years have been an important subject of interest. Casein micelles are ...

High protein dairy beverages are considered to be mouth drying. The drying sensation may be due to the product protein content; however the mechanism of this mouth drying is uncertain. This study investigated the potential adhesion of milk proteins to porcine oral mucosa in vitro. Purified casein and β-lactoglobulin were fluorescently labelled, placed on porcine oral mucosal tissues and their r...

Wedholm, A. 2008. Variation in milk protein composition and its importance for the quality of cheese milk. Doctoral dissertation. IS 978-91-85913-46-6, ISSN: 1652-6880 Variations within the bovine milk protein profile can have both positive and negative impacts on the quality of cheese milk. As main contributors to the cheese structure, the caseins are important for the cheese yield. During the...

Epithelial cells from mammary gland tissue that are cultured in vitro are able to maintain specific functions of this gland, such as cellular differentiation and milk protein synthesis. These characteristics make these cells a useful model to study mammary gland physiology, development and differentiation; they can also be used for production of exogenous proteins of pharmaceutical interest. Bo...

Quantitative RT-PCR analysis. The following primers were used to amplify β-casein cDNA and 18S rRNA sequences: forward primer of the β-casein gene 5'-GCT CAG GCT CAA ACC ATC TC-3' and reverse primer 5'-TGT GGA AGG AAG GGT GCT AC-3'; forward primer of the 18S rRNA gene 5’-TCGGAACTGAGGCCATGATT-3’ and reverse primer 5’CCTCCGACTTTCGTTCTTGATT-3’. Fragments of β-casein gene and 18S rRNA were amplifie...

The literature review is devoted to biologically active metabolites of casein — the results its hydrolysis oligopeptides casomorphins. These peptides with a chain length 4 11 amino acids are derived from milk β-casein and released during digestion, both in vivo vitro. Caseomorphins exhibit opioid pharmacological activity due binding μ-receptors located central nervous system, gastrointestinal t...

Pten is a tumor suppressor gene regulating many cellular processes, including growth, adhesion, and apoptosis. In the aim of investigating the role of Pten during mammary gland development and lactation of dairy cows, we analyzed Pten expression levels in the mammary glands of dairy cows by using western blotting, immunohistochemistry, and quantitative polymerase chain reaction (qPCR) assays. D...

the proline-rich β-casein was digested in vitro with trypsin, and the oligopeptides producedwere then isolated by rp-hplc and subsequently identified by amino acid analysis and ion massspectrometry. the peptide fractions from the complete digestion were then treated with purified x-prolyldipeptidyl peptidase (x-pdp) extracted from lactobacillus casei ssp. casei llg. two bitter peptides (f53-97a...

Biochemical, volatile and textural profiles during manufacture and ripening were determined in samples of Castelmagno PDO cheese obtained from three different batches in the main artisan cheese plant of Castelmagno PDO production area. At the end of manufacture, samples were characterised by a pH of 6.57% and 52.4% moisture content. The HPLC analysis of organic acids and sugars showed the exhau...