At least 8 macromolecules in the supernatant fraction of rat liver immunologically cross-react with antigens extracted from liver microsomes. These supernatant analogs of microsomal antigens have been resolved by extensive column electrophoresis and differentially characterized. One species («)is highly acidic, 3 (ß, TI, and y2) are of intermediate acidity, and 4 (*, ei, 62>and Õ)are near-ne...

Galectin-3 is a ß-galactoside-specific lectin implicated in diverse pro cesses involved in cellular interactions. Recently, the Mac-2-binding pro tein, a heavily /V-glycosylated secreted protein with a subunit MTof 97,000, was identified as its ligand. The present study characterizes the interaction between galectin-3 and Mac-2-binding protein in whole cells and measures their relative express...

DL-f/7reo-j8-Fluoroasparagine (DL-f/weo-/3-F-Asn) inhibited the growth of murine leukemia L1210 cells and three human leuke mia cell lines in culture. Fifty % inhibiting dose values ranged between 30 and 50 MMDL-fftreo-^-F-Asn. L1210 cells were not sensitive to DL-eryf/jro-/8-fluoroasparagine, DL-f/veo-/3-fluoroaspartic acid, or DL-e/yf/»ro-/3-fluoroaspartic acid at 300 MM, the highest dose st...

A subset of salivary epithelial tumors induced by mouse polyoma virus (PyV) has been designated lymphoepithelioma on the basis of a prominent lymphocytic component. Serial transplantation of this variant has previ ously been observed to result in lymphoma development. A recent repe tition of this phenomenon allowed us to characterize the lymphoma cell populations with regard to phenotypic marke...

We cultured primary human mammary epithelial cells from five re duction mammoplasties and five breast carcinomas and attempted to improve culture conditions and define cell populations grown. Normal cells cultured on Matrigel basement membrane-like substance formed multicellular three-dimensional structures reminiscent of tissue ducts and alveoli, while malignant cells remained as single cells ...

Ectopie expression of Carcinoembryonic antigen (CEA) by the mela noma cell line Mel Wei led to alterations in cell morphology and to changes in the expression of two melanoma-associated cell adhesion mol ecules, neural cell adhesion molecule and MUC18. The normally flat, triangular cells developed neurite-like extensions and exhibited a less organized growth pattern. When compared to untransfec...

The effect of A/-[phosphonacetyl]-L-aspartate (PALA) on the metabolism and cytotoxicity of 1-/S-o-arabinofuranosylcytosine (ara-C) was studied in the human promyelocytic leukemic cell line, HL-60, and in normal human bone marrow. HL-60 cells exposed to 0.1 mw PALA for 12 hr accumulated 58.7 pmol ara-C per 106 cells after a 45-min exposure to 1 /XMara-C, compared to 27.8 pmol ara-C per 106 cells...

background: generally, a number of people with î²-thalassemia are susceptible to hbv since they receive multiple blood transfusions. these people have high infection rates despite vaccination against hbv. in this study, we explore the seroprevalence and risk factors of hbv infection in thalassemic patients who reside in ardabil. materials and methods: hospital in ardabil. patients' demographic ...

p53 induction and cell cycle arrest occur following DNA damage, possibly to allow repair prior to replication. p21WAFI/CIP11a cyclin-cyclindependent kinase inhibitor and proliferating cell nuclear antigen-inter acting protein, is induced by p53 and mediates the cell cycle arrest. To investigate a role for p21 in DNA repair in vivo, we studied the expression of in vitro damaged reporter DNA tran...

In the studies presented L1210 leukemia was used to show that various equipotent combinations of two drugs can be freely substituted for each other during the course of sequential treatment with other drugs and that the order of administration will influence the therapeutic re sponse to sequential treatment with various drug combi nations. Mice inoculated i.p. with L1210 ascites tumor cells wer...