SO2 is traditionally used to limit or nullify the proliferation of spoilage yeasts in must/wine, but its use has become controversial due negative effects on human health. An alternative strategy for control biocontrol yeasts. Wickerhamomyces anomalus an outstanding biocontroller yeast that been examined by our group and a good agent against Brettanomyces bruxellensis Zygosaccharomyces rouxii. ...

Saccharomyces cerevisiae M30, a high ethanol producing flocculent strain, was hybridized with Zygosaccharomyces rouxii TISTR1750, a halotolerant non-flocculent strain, by polyethylene glycol induced protoplast fusion. The protoplast fusion of these two yeast genera occurred with frequency of 5.89X10-5. Three fusants, namely RM11, RM37 and RM58, were selected based on their high ethanol fermenta...