Traditionally measurements of service quality have followed the expectation-disconfirmation approach. Further, previous studies shown that negative disconfirmation is more influential than positive disconfirmation. In this research, we hypothesized information systems(IS) scales based on dimensionality (ED) and desire-disconfirmation (DD) Using SERVQUAL+ instrument data collected from 321 IS us...

Four pathogenic Vibrio species were isolated from three diseased black rockfish Sebastes schlegeli in Yantai, Shandong Province, China. The strains identified based on physiological and biochemical characteristics 16S rDNA sequencing named SF-2, SF-3, SF-5, SF-6, respectively. SF-2 was scophthalmi, SF-3 V. harveyi, SF-5 alginolyticus, SF-6 parahaemolyticus. This is the first time that scophthal...

A total of 124 V cholerae non-O1/non-O139 isolates were collected in Khon Kaen, Thailand from diarrheal patients, asymptomatic carriers and environmental water. The presence of virulence-associated and regulatory genes including ctxA, tcpA, zot, ace, ompU, stn, hlyA and toxR) were examined using multiplex PCR. The genomic diversity of the various V. cholerae isolates were differentiated using t...

Background: Stenotrophomonas maltophilia is an opportunistic bacterium, contributing to different hospital-acquired infections and can be acquired from different hospital setting sources. Epidemiological study of S. maltophilia in the hospital also demonstrates the intrahospital distribution of certain strains of bacteria in healthcare facilities. The aim of the current study was to identify th...

V. cholerae non-O1 non-O139 serogroups isolated from clinical and environmental sources in Córdoba, Argentina, were analyzed for the presence and expression of virulence genes. Most of the strains studied contained the genes toxR and hlyA, but lacked ctxA, zot, ace, tcpA and stn. The culture supernatants were tested for hemolytic and cytotoxic activity. The enterotoxic potential of the strains ...

One hundred seventy nine Vibrio cholerae non-O1/non-O139 strains from clinical and different environmental sources isolated in Brazil from 1991 to 2000 were serogrouped and screened for the presence of four different virulence factors. The Random Amplification of Polymorphic DNA (RAPD) technique was used to evaluate the genetic relatedness among strains. Fifty-four different serogroups were ide...

OBJECTIVE The bacterium Oceanimonas sp. (O. sp.) GK1 is a member of the Aeromonadaceae family and its genome represents several virulence genes involved in fish and human pathogenicity. In this original research study we aimed to identify and characterize the putative virulence factors and pathogenicity of this halotolerant marine bacterium using genome wide analysis. MATERIALS AND METHODS Th...

This paper presents a verification technique for dense-time MTL based on discretization. The technique reduces the validity problem of MTL formulas from dense to discrete time, through the notion of sampling invariance, introduced in previous work [13]. Since the reduction is from an undecidable problem to a decidable one, the technique is necessarily incomplete, so it fails to provide conclusi...

Vibrio cholerae is the etiological agent of epidemic cholera. Symptoms include stomach ache, bloody diarrhea, and vomiting, which lead to severe dehydration and even death. Environmental monitoring as well as rapid and accurate identification of this pathogen are important for public health protection. In this study, a real-time PCR method for the detection of toxigenic V. cholerae was develope...