The intracellular localization of Cu,Zn superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) has been examined by immunofluorescence using four monoclonal anti-Cu,Zn superoxide dismutase antibodies raised against a recombinant human Cu,Zn superoxide dismutase derivative produced and purified from Escherichia coli. Colocalization with catalase, a peroxisomal matrix enzyme, wa...

Neuronal-growth-inhibitory factor (GIF) is a metalloprotein specific to the central nervous system which has been linked to Alzheimer's disease. The high metal content, approximately seven metal atoms/protein molecule, and 70% sequence identity to mammalian metallothioneins (MT), including a preserved array of 20 cysteinyl residues, place GIF in the family of MT. In contrast to MT, native GIF i...

Aim: To assess serum Cu/Zn SOD (Superoxide Dismutase) concentration in individuals with bipolar disorder. Subjects and methods: Serum from 20 individuals diagnosed with bipolar disorder and 20 age and gender similar controls were tested for Cu/Zn SOD serum concentration using ELISAs. Results: Serum Cu/Zn SOD levels of individuals with bipolar disorder were significantly higher than age and gend...

during estuarine mixing of fresh water with saline water and due to the flocculation process, aportion of dissolved metals come into particulate phase, and the dissolved load decreases. this process plays an important role in self-purification of heavy metals in rivers. in this study, flocculation of cu, mn, ni, pb and zn during mixing of cheshme-kileh river water with caspian sea water has bee...

Determination and recognition of relative distribution of chemical forms of each element and their relationship with physical, chemical and soil clay minerals can help researchers to manage soil fertility better. This research attempted to recognize chemical fractions of zinc (Zn) and copper (Cu) in some surface and subsurface soil samples of Kohgiluyeh and Boyer-Ahmad province and their relati...

One hundred twenty male Sprague-Dawley rats, averaging 108.6 g initial body weight, were used in two feeding experiments to evaluate the utilization of Cu in Cu proteinate, Cu lysine, and cupric sulfate. In Exp. 1, 60 rats were randomly assigned to 12 treatments in a 2 x 2 x 3 factorial arrangement of treatments, with Zn supplementation at 0 or 1000 mg/kg diet, Cu supplementation at 5 or 15 mg/...

Amyotrophic lateral sclerosis has been linked to the gain of aberrant function of superoxide dismutase, Cu,Zn-SOD1 upon protein misfolding. The mechanism of SOD1 misfolding is thought to involve mutations leading to the loss of Zn, followed by protein unfolding and aggregation. We show that the removal of Zn from SOD1 may not lead to an immediate unfolding but immediately deactivates the enzyme...

The batch sorption capability of Romanian pine bark for the removal of Cu(II) and Zn(II) ions from diluted aqueous solutions was investigated as a function of initial pH, contact time, and temperature. The metal sorption sequence is Cu > Zn. The experimental data for Cu(II) and Zn(II) ions retention on the tested bark of Pinus sylvestris L. showed a better compliance with the pseudo–second orde...