Aminoacylase was identified in cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus by its ability to hydrolyze N-acetyl-L-methionine and was purified by multistep chromatography. The enzyme is a homotetramer (42.06 kDa per subunit) and, as purified, contains 1.0 +/- 0.48 g-atoms of zinc per subunit. Treatment of the purified enzyme with EDTA resulted in complete loss of activity...

Cadmium (Cd) is a highly toxic heavy metal that is naturally present in the environment. Chronic exposure to Cd causes hepatotoxicity and nephrotoxicity. The present study aimed to study the protective effect of melatonin, methionine and zinc against histopathological, histochemical and proliferative effects of cadmium on the kidney of rats. A total of 80 female albino rats were included in thi...

Essential amino acids such as methionine have been increasingly included into diets of lambs. However, few studies have determined the effects of methionine supplementation in the general performance and economic returns of lamb production. To address this question, 21weaned male Rahmani lambs were allotted to 3 dietary treatments: control diet (C), control diet plus 3.30 g of methionine/kg con...

this research was conducted to evaluate the effects of synthetic methionine replacement with herbal methionine (hm) on performance, blood biochemical parameters and carcass characteristics in broiler chicken. a total of 280 8-old ross 308 male chicken were randomly assigned to 7 dietary treatments: control, in which 100% methionine requirement was supplied form dl-methionine, and in treatments ...

Various factors that are important for proper hearing have been identified, including serum levels of zinc. Here we investigated whether epigenetic regulatory pathways, which can be modified by environmental factors, could modulate hearing. RT-PCR detected expression of genes encoding DNA methyltransferase and histone deacetylase (Hdac) in the postnatal as well as adult mouse auditory epitheliu...

background: the degree to which the amount of aningested nutrient is absorbed and available to the body is calledbioavailability. objectives: relative effectiveness of herbalmethionine (h-met®) compared to dl-methionine (dl-met)was investigated in this experiment. methods: exponentialregression analysis was used to determine bioefficacy of h-met®based on body weight gain, feed intake and feed c...

Abstract The precise regulatory role in protein synthesis by facilitating interactions with mRNA codons for various tRNA modifications is unclear. We previously reported that enhanced green fluorescent (GFP) reduced GFP expression human methionine-conjugated initiator (tRNAi)/tRNA synthetase pairs under methionine-deficient conditions. Here, we investigated the effect of non-formylated Escheric...

Background: The search for potent radioprotective agents for the amelioration of radiation side effect is an important aim in radiobiology. The present study aimed to evaluate the effects of curcumin and seleno-L-methionine against radiation-induced micronucleus formation in rat bone marrow.Methods: In total, 40 male rats were divided into 8 groups (n=5 each), including control, curcumin or sel...