Lymph nodes from guinea pigs inoculated with Mycobacterium tuberculosis were fixed in buffered formalin, then treated for the recommended times in Gooding and Stewart's fluid, EDTA, aqueous nitric acid, von Ebner's fluid, and rapid decalcifier (RDC). The blocks were processed to paraffin wax and sections were stained by the Ziehl-Neelsen technique. Only in sections of the blocks treated with RD...

Negative staining technique of Heine is an easy, inexpensive, and rapid way of screening for coccidian parasites of the intestinal tract. But its use as a routine technique for screening of Cryptosporidium, Cyclospora, and Cystoisospora is restricted due to its sensitivity being lower than the gold standard method of modified Ziehl-Neelsen staining. This paper emphasises the modification of ori...

The authors studied 66 wild nine-banded armadillos from Brazil. The ear samples were collected and Ziehl-Neelsen or Fite-Faraco stains were performed, as well as immunostaining using polyclonal BCG antibody, to avaluate the presence of the Mycobacterium leprae. The AFB were not detected by the Ziehl-Neelsen or Fite-Faraco staining, neither immunoexpression of the BCG marker. However, many norma...

We analyzed 150 patients with pulmonary tuberculosis from 1990 to 1996 (i) to evaluate the frequency of drug resistance, (ii) to elucidate factors influencing the response to chemotherapy, and (iii) to attempt to improve the therapeutic approach. Multidrug-resistant tuberculosis strains were not found. By univariate analysis, there were 8 factors associated with an increased sputum conversion t...

Background: Cryptosporidium has emerged as an important cause of diarrheal illness worldwide, especially among young children and patients with immune deficiencies. The spectrum of intestinal infection ranges from asymptomatic carrier state to severe diarrhea, depending upon the nutritional and immune status of the host. Materials & Methods: 387children, in the age group from 1 month to 10 year...

To compare the sensitivity of 2 microscopic methods for diagnosis Mycobacterium tuberculosis (M.tb) along with culture and drug susceptibility testing to first line drugs. : The cross-sectional study comprises 200 suspected cases pulmonary both clinically radiologically in KIMS, Bangalore over a period years. Samples (sputum/BAL fluid) were collected, processed stained by Ziehl Neelson (ZN) Flu...

Faecal samples from 291 dairy calves from 10 dairy herds in Kerman were examined to investigate theprevalence of Cryptosporidium infection. Cryptosporidium spp. oocysts were identified by using themodified Ziehl-Neelsen staining technique in 63 (21.65%) calves. Calves were grouped according to theirage as follows: