Over the last decade, zebrafish has proven to be a powerful model in cancer research. Zebrafish form tumors that histologically and genetically resemble human cancers. The live imaging and cost-effective compound screening possible with zebrafish especially complement classic mouse cancer models. Here, we report recent progress in the field, including genetically engineered zebrafish cancer mod...

The rise of zebrafish as a neuroscience research model organism, in conjunction with recent progress in single-cell resolution whole-brain imaging of larval zebrafish, opens a new window of opportunity for research on interval timing. In this article, we review zebrafish neuroanatomy and neuromodulatory systems, with particular focus on identifying homologies between the zebrafish forebrain and...

This protocol describes regular care and maintenance of a zebrafish laboratory. Zebrafish are now gaining popularity in genetics, pharmacological and behavioural research. As a vertebrate, zebrafish share considerable genetic sequence similarity with humans and are being used as an animal model for various human disease conditions. The advantages of zebrafish in comparison to other common verte...

As one of three akt isoforms, akt2 plays a key role in the regulation of widely divergent cellular processes in mammals. However, its role and underlying mechanisms in zebrafish remain largely unknown. To elucidate the function of akt2 in zebrafish, we generated zebrafish lacking akt2 gene via CRISPR/Cas9 technology. Akt2-null zebrafish exhibit partial lethality and severe growth deficiency, wh...

Leptin is a hormone which functions in the regulation of energy homeostasis via suppression appetite. In zebrafish, there are two paralogous genes encoding leptin, called lepa and lepb . gene expression study, we found that gene, not , was significantly downregulated under state insulin-resistance zebrafish larvae, suggesting plays role glucose homeostasis. current characterised -deficient ( −/...

The use of the visible implant elastomer (VIE) tagging system in zebrafish (Danio rerio) was examined. Two tag orientations (horizontal and vertical) at the dorsal fin base were tested for tag retention, tag fragmentation and whether VIE tags affected growth and survival of juvenile zebrafish (1-4 month post hatch). Six tag locations (abdomen, anal fin base, caudal peduncle, dorsal fin base, pe...

Zebrafish epidermal ionocytes are analogous to mammalian kidney cells in terms of expression and function of ion transporters. In this review, we summarize current findings about the development of the zebrafish epidermis and demonstrate how the zebrafish regulate stress acclimation through induction of cell differentiation. In addition, cellular homologies between zebrafish epidermal ionocytes...

The need of aquarists for proper and safe disinfection of fish with Chloramine-T (CL-T) necessitates toxico/pathologic examinations.   This study assays toxicity level of CL-T and histopathologic changes of gills, liver, kidney and brain of treated zebrafish (Danio rerio). Groups of 10 Zebrafish were treated with 0 (control), 5, 10, 15, 30, 50,100 and 200 mg/L of CL– T. Mortality was recorded a...

The zebrafish (Danio rerio) is a powerful model organism for the study of vertebrate biology, being well suited to both developmental and genetic analysis. More recently, the rapid progress of various zebrafish genomics infrastructure initiatives is facilitating the development of zebrafish models of human disease. Genome organization and the pathways involved into control of signal transductio...