We discovered six plant extracts that increase yeast chronological lifespan to a significantly greater extent than any of the presently known longevity-extending chemical compounds. One of these extracts is the most potent longevity-extending pharmacological intervention yet described. We show that each of the six plant extracts is a geroprotector which delays the onset and decreases the rate o...

Green tea (GT), cranberry (CR), and tart cherry extracts were evaluated for their ability to inhibit yeast α-glucosidase, relevant to glucose uptake. The total phenolic content (TPC), antioxidant activity, and in vitro inhibitory activity of yeast α-glucosidase were examined for the extracts in the present study. GT had higher TPC and antioxidant activity, but CR demonstrated a greater α-glucos...

Activating phosphorylation of cyclin-dependent protein kinases (CDKs) is necessary for their kinase activity and cell cycle progression. This phosphorylation is carried out by the Cdk-activating kinase (CAK); in contrast, little is known about the corresponding protein phosphatase. We show that type 2C protein phosphatases (PP2Cs) are responsible for this dephosphorylation of Cdc28p, the major ...

Knowledge concerning the nature of the biologic synthesis of purine or pyrimidine mononucleotides is relatively limited. A significant contribution was the demonstration by Ostern and coworkers (3) of the phosphorylation of adenosine to adenosine-5’-phosphate (A-5’-P) and adenosinetriphosphate (ATP) by means of crude yeast extracts. The energy source for this reaction was supplied by the fermen...

A large number of medicinal plants have therapeutic potentials. In this study polyphenolic extracts from leaves of Wattakaka volubilis have been applied for HPLC, which reports the presence of seven flavonoids. Extracts have been tested for antimitotic and antiproliferating activities. Allium cepa root tips were been used to evaluate cytotoxicity. Polyphenolic and methanolic extracts were toxic...

Rab GTPases participating in the regulation of vesicle trafficking in eukaryotes are geranylgeranylated by the Rab geranylgeranyl transferase (RabGGTase) in complex with the Rab escort protein (REP). Here, we describe basic properties of the Arabidopsis thaliana REP (AthREP), first REP outside yeasts or metazoans to be characterized. GFP-tagged AthREP, as well as the geranylgeranylation activit...

The yeast transcriptional activator ADR1, which is required for ADH2 and other genes' expression, contains four transactivation domains (TADs). While previous studies have shown that these TADs act through GCN5 and ADA2, and presumably TFIIB, other factors are likely to be involved in ADR1 function. In this study, we addressed the question of whether TFIID is also required for ADR1 action. In v...

The main objective of this study was to analyze the growth kinetics milk kefir on bean sprouts (Glycine max) as a germinated substrate for 4 days by glass jar method followed extracts. Ratio 1:3 (soy: water). 3% grains (activated) inoculated with soluble bud extract has total sugar content 5.57% and fermentable carbohydrate 3.37 g / L (sucrose), which is source nutrition energy reserves; 1.40% ...