Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as the protein source. Beef extractives and starch are not added. Activated charcoal (Norit A or Norit SG) is included at 0.20% ...

A newly isolated fungus Aspergillus niger SOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation...

Polyvinyl alcohol-degrading enzymes (PVAases) have a great potential in bio-desizing processes for its low environmental impact and low energy consumption. In this study, the effect of yeast extract on PVAases production was investigated. A strategy of four-point yeast extract addition was developed and applied to maximize cell growth and PVAases production. As a result, the maximum dry cell we...

Sinorhizobium meliloti is usually cultured in rich media containing yeast extract. It has been suggested that some components of yeast extract are also required for growth in minimal medium. We tested 27 strains of this bacterium and found that none were able to grow in minimal medium when methods to limit carryover of yeast extract were used during inoculation. By fractionation of yeast extrac...

The present study was aimed to use spent wash as a substitute for expensive commercial media for Rhizobial cultures. Growth of Rhizobium strains at different concentrations of spent wash was observed and found maximum at 50% spent wash. Bacteria were grown on 50% spent wash which produced remarkable results and competed with standard media like tryptone yeast extract, Rhizobium minimal and yeas...

The anticandidal activity of the methanolic extract of Wedelia chinensis leave was studied and tested against four pathogenic Candida albicans strains of clinical isolates. The extract exhibited favorable anticandidal activity with the zones of inhibition between 18.00 ± 0.10 to 20.00 ± 0.17 mm and minimum inhibition concentration (MIC) values between 3.13 and 6.25 mg/mL. The time-kill studied ...

To study the import of proteins into mitochondria we developed a homologous in vitro system in which mitochondria and cell-free translation extract are both derived from the yeast Saccharomyces cerevisiae. This system allows the synthesis of precursor proteins in the presence of isolated mitochondria and offers a means of analyzing yeast mutants defective in mitochondrial protein import. The in...