Trichromatic colorimetry was applied for the evaluation of the colour changes of the metallochromic indicators, viz., hematoxylin, gallocyanine, Gallamine Blue, Solochrome Prune AS, Celestine Blue, Eriochrome Black T, Xylenol Orange and Catechol Violet, in the complexometric titration of zirconium( IV) with ethylenediaminetetraacetic acid (EDTA) to measure the quality of colour change at the eq...

I describe the characterization, extraction, and purification of a cholesterol:oxygen oxidoreductase (EC 1.1.3.6) from Nocardia sp. This enzyme catalyzes oxidation of cholesterol to z4-choIestenone, with production of hydrogen peroxide. It is very stable, active over a wide pH range, and has a Km of 1.4 X i0 mol/liter. It is highly specific for or 3f3-hydroxycholestanes, and may be applied to t...

A simple and sensitive method is presented for the simultaneous quantification (spectrophotometric and spectrofluorimetric) of the main lipid and protein peroxidation products after their initial fractionation: free malondialdehyde (FrMDA), protein-bound malondialdehyde (PrMDA), total hydroperoxides (LOOH), and protein hydroperoxides (PrOOH). FrMDA and PrMDA (released from proteins by alkaline ...

Spectral data during a chelatometric titration were utilized for determining an equivalence point of the titration. Three-dimensional titration data were analyzed by fitting to a theoretical equation that was derived from equilibrium equations among a metal ion, an indicator and EDTA. The equation with optimized parameters successfully explained the three-dimensional titration data. This method...