The fungus Cryptococcus neoformans possesses a polysaccharide capsule and can form biofilms on medical devices. We describe the characteristics of C. neoformans biofilm development using a microtiter plate model, microscopic examinations, and a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium-hydroxide (XTT) reduction assay to observe the metabolic...

This paper presents advances in Set Attributive Logic and its application to develop tabular rule-based systems within the XTT framework. The primary goal is to extend the expressive power of simple attributive languages so that it becomes satisfactory for complex applications, including the business rules support. A formal framework of extended Attributive Logic with Set Values over Finite Dom...

This paper presents proposals of certain extensions to the XTT knowledge representation model, a method of tabular specification for rule-based systems. The extensions concern introduction of variables, functionally dependent attributes, constraints, fuzzy rules and time-delayed values.

Objective: This study aimed to assess the enhanced anticancer effect of Temozolomide (TMZ) through a synergistic combination with Diltiazem (DTZ) in neuroblastoma cell line (SH-SY5Y).
 Material and Methods: The SH-SY5Y was cultured DMEM medium. TMZ DTZ stock solutions were prepared diluted obtain different concentrations. XTT assay performed evaluate viability. Cells treated alone or for 2...

A colorimetric assay for antifungal susceptibility testing of Aspergillus species (Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, Aspergillus nidulans, and Aspergillus ustus) is described based on the reduction of the tetrazolium salt 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2H-tetrazolium-hydroxide (XTT) in the presence of menadione as an electron-coupling agent....

A practical design of non-trivial rule-based systems requires a systematic, structured and consistent approach. The paper focuses on selected issues in RBS knowledge engineering. Some ideas on combining knowledge engineering with software engineering are discussed. Furthermore, results of RBS design tools survey are enclosed. In the paper an original design and implementation methodology for RB...

BACKGROUND opioid and somatostatin receptors (SSTRs) that can assemble as heterodimer were individually reported to modulate malignant cell proliferation and to favour apoptosis. MATERIALS AND METHODS SSTRs and opioid receptors expression were examined by RT-PCR, western-blot and binding assays, cell proliferation was studied by XTT assay and propidium iodide (PI) staining and apoptosis by an...

This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulte...