Wnt/b-catenin signalling regulates cell proliferation by modulating the cell cycle and is negatively regulated by conductin/axin2/ axil. We show that conductin levels peak at G2/M followed by a rapid decline during return to G1. In line with this, Wnt/b-catenin target genes are low at G2/M and high at G1/S, and b-catenin phosphorylation oscillates during the cell cycle in a conductindependent m...

We developed a slow structural relaxation model to describe cellular dynamics in the crypt of the mouse small intestine. Cells are arranged in a three dimensional spiral the size of which dynamically changes according to cell production demands of adjacent villi. Cell differentiation and proliferation is regulated through Wnt and Notch signals, the strength of which depends on the local cell co...

Wnt/β-catenin signalling regulates cell proliferation by modulating the cell cycle and is negatively regulated by conductin/axin2/axil. We show that conductin levels peak at G2/M followed by a rapid decline during return to G1. In line with this, Wnt/β-catenin target genes are low at G2/M and high at G1/S, and β-catenin phosphorylation oscillates during the cell cycle in a conductin-dependent m...

hepatitis b virus (hbv) is considered as a global health concern and hepatitis b surface antigen (hbsag) is the most immunogenic protein of hbv. the purpose of this study was to evaluate the expression of hbsag on the cell surface of human embryonic kidney cell line (hek293t). after transformation of expression vector pcdna/hbsag to e.coli top10f’, plasmid was extracted and digested with bglii....

Wnt signalling pathways play crucial roles in developmental biology, stem cell fate and tissue patterning and have become an attractive therapeutic target in the fields of tissue engineering and regenerative medicine. Wnt signalling has also been shown to play a role in human Mesenchymal Stem Cell (hMSC) fate, which have shown potential as a cell therapy in bone and cartilage tissue engineering...

BACKGROUND Osteoblasts are bone forming cells that play an essential role in osteogenesis. The elucidation of the mechanisms that control osteoblast number is of major interest for the treatment of skeletal disorders characterized by abnormal bone formation. Canonical Wnt signalling plays an important role in the control of osteoblast proliferation, differentiation and survival. Recent studies ...

Abstract Background Crohn’s disease (CD) is characterized by chronic recurrent intestinal inflammation that leads to progressive fibrotic remodelling of the wall. Despite advances in medical therapy for CD, 50% patients still require surgical treatment after 10 years mainly due fibrosis-associated stenosis formation. A specific anti-fibrotic does not yet exist, underlining need further research...

background: natural medicines have been recently considered more reasonable for human use most notably due to their safety and tolerance. hesa-a is a marine-originated herbal medicine with a variety of healing effects. however, its exact biological mechanism is not clear. the pre-sent study aimed at the evaluation of the hesa-a antioxidant effect. methods: chinese hamster ovary (cho) and human ...

The retinal pigment epithelium (RPE) is indispensable for vertebrate eye development and vision. In the classical model of optic vesicle patterning, the surface ectoderm produces fibroblast growth factors (FGFs) that specify the neural retina (NR) distally, whereas TGFβ family members released from the proximal mesenchyme are involved in RPE specification. However, we previously proposed that b...

Low-density lipoprotein receptor-related proteins 5 and 6 (LRP5/6) function as transmembrane receptors to transduce Wnt signals. A key mechanism for signalling is Wnt-induced serine/threonine phosphorylation at conserved PPPSPxS motifs in the LRP6 cytoplasmic domain, which promotes pathway activation. Conserved tyrosine residues are positioned close to all PPPSPxS motifs, which suggests they ha...