Enteric fever is a major public health problem in developing countries like India. An early and accurate diagnosis is necessary for a prompt and effective treatment. We have evaluated the diagnostic accuracy of ENTEROSCREEN-WB as compared to Widal test in rapid and early diagnosis of enteric fever. A total of 145 patients serum samples were tested by Rapid ENTEROSCREEN-WB and Widal test includi...

AIM To determine the possibility of leptospirosis among patients from urban slums presenting with febrile illness during monsoon and post-monsoon season. METHODS Evidence of leptospirosis in 180 patients with febrile illness was determined by looking for presence of immunoglobulin M (IgM) antibodies by leptospiral IgM enzyme linked immunosorbent assay (ELISA). The test was carried out on 160 ...

AIMS To compare the established Widal agglutination assay with an immunoblotting procedure. METHODS 110 sera were used to compare the established Widal agglutination assay with an immunoblotting procedure incorporating lipopolysaccharide (LPS) (O = 9,12) and flagellar (H = d) antigens. RESULTS Antibodies to the LPS antigens were detected in 18 sera by the Widal assay and in 37 by immunoblot...

Typhoid fever is an infection caused by a strain of bacteria called Salmonella typhi, which is related to the bacteria that causes salmonella food poisoning. The infection can affect the whole body and damage multiple organs. Unless treated, this infection can have life threatening consequences. A 20 year old girl was examined suffering from high fever from one week and abdominal pain for few d...

While carrying out routine Widal tests at the Pasteur Institute Laboratory, Shillong, we met from time to time with specimens from patients in Assam whose history suggested enteric fever, but whose serum gave Widal reactions not in the least indicative of that disease, even when repeated two or three times at weekly intervals. Whilst this, of course, is a common enough experience in any laborat...

Introduction: Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations. Methodology: Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses ...

Estimates for the year 2000 suggested that there were approximately 21.5 million infections and 200,000 deaths from typhoid fever globally each year, making the disease one of the most serious infectious disease threats to public health on a global scale. However, these estimates were based on little data, especially from Africa. Global prominence and high-profile outbreaks have created the per...

INTRODUCTION Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations. METHODOLOGY Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses ...