We present a microfluidic platform for performing fast, continuous flow DNA hybridisation on magnetic particles. Laminar flow streams containing sample oligonucleotides, washing buffer, fluorescently labelled oligonucleotides and washing buffer, were generated in the chamber of a microfluidic chip. Magnetic particles were then introduced into the chamber, and pulled through each of the streams ...

Recent advances in nanoscience have led to the development of numerous methodologiesfor controlled synthesis of mono dispersed nanoparticles and/or nanoclusters via surface stabilization by organic capping ligands. The application of these nanoparticles in catalysis and other fields often requires the removal of organic ligands. It is known that the removal of organic capping agents or or...

the chemical extraction of twenty-nine elements in a washing aggregate sludge, a clay-richsediment, a sewage sludge, the mixtures of these materials and the artificial lightweight aggregates (lwas)manufactured with them was determined by applying the optimized european community bureau of reference sequential extraction procedure in order to establish the effect of the thermal treatment on the ...

AIMS To investigate treatment with glycerol/washing as a potential substitute for freeze-thawing in the production of leucocyte depleted red cell concentrates for patients with a history of non-haemolytic reactions following transfusion. METHODS The standard procedure of treatment with glycerol/-80 degrees C freezing/thawing/washing was compared with a similar procedure in which freezing was ...

BACKGROUND Oxygen desaturation is not uncommon during bronchoscopy. We sought to identify factors predictive of desaturation during flexible bronchoscopy. METHODS Over eight months, we enrolled 137 randomly selected patients who were undergoing fiberoptic bronchoscopy at our medical center. The patients' oxygen saturation was monitored on their arrival and during the procedure by finger pulse...

INTRODUCTION Live peripheral blood mononuclear cells (PBMCs) can be frozen and thawed for later analyses by adding and removing a cryoprotectant, such as dimethyl sulfoxide (DMSO). Laboratories across the world use various procedures, but published evidence of optimal thawing procedures is scarce. MATERIALS AND METHODS PBMCs were separated from blood collected from healthy Danish blood donors...

BACKGROUND Determining levels of tumor markers in peritoneal washing enables likelihood of peritoneal recurrence to be ascertained in patients with high marker levels, thereby allowing provision of more accurate adjuvant treatment and postoperative follow up. AIM To analyze the relationship between levels of tumor marker CA72-4 in serum and peritoneal washing, and morphological aspects of gas...

Textile materials from polyester fibres are sensitive to washing, especially at higher temperatures, due their thermoplastic properties, hydrophobic nature and sensitivity the alkaline medium. The issue of microplastic fibres’ (MFs’) release textiles is a topic that attracts attention researchers different scientific fields, since microplastics now among serious environmental risks. In this stu...

Introduction Many items are needed for dressing including sterile dressing set, antiseptic and washing solutions, leucoplast tape, waste bin for infectious garbage, waste bin for noninfectious garbage, safe disposal trash for sharp cutting instruments, bedpan and sometimes drugs. All the items are laid out on a simple wheeled trolley. The multiplicity of items together with problems in placing ...