We describe a comparison of the scrub-wash method of Williamson and Kligman and a swab-wash method for the enumeration of cutaneous microflora. The swab-wash method provides a less traumatic alternative to the scrub-wash method and can be used to sample the cutaneous microflora of premature neonates.

This article describes the clinical procedures for a modified putty-wash impression technique. In this method, the hydraulic pressure induced from planned flowing the wash material from the inner surface of putty impression toward the vestibules pull over the gingival tissues covering the finishing line. This simple method reduces the need to using retraction cord in normal depth finishing line...

Subtelomeres are duplication-rich, structurally variable regions of the human genome situated just proximal of telomeres. We report here that the most terminally located human subtelomeric genes encode a previously unrecognized third subclass of the Wiskott-Aldrich Syndrome Protein family, whose known members reorganize the actin cytoskeleton in response to extracellular stimuli. This new subcl...

There is increasing evidence that cytoskeleton remodeling is involved in cancer progression. Wiskott-Aldrich syndrome protein (WASP) family represents a key regulator of actin cytoskeleton remodeling. However, the underlying mechanism of the WASP family in cancer progression remains elusive. Here, we studied the role of WASP and SCAR Homolog (WASH), a recently identified WASP family member, in ...

The Wiskott-Aldrich syndrome protein and SCAR homolog (WASH; also known as Washout in flies) is a conserved actin-nucleation-promoting factor controlling Arp2/3 complex activity in endosomal sorting and recycling. Previous studies have identified WASH as an essential regulator in Drosophila development. Here, we show that homozygous wash mutant flies are viable and fertile. We demonstrate that ...

PURPOSE We conducted a comparative study to optimize the scanning and inhalation protocols for xenon CT cerebral blood flow (CBF) examination (Xe CT), with the aim of improving the practical performance of Xe CT as a routine clinical examination. MATERIALS AND METHODS Four different inhalation protocols, including 3-min, 6-min, and 8-min wash-in protocols, and a 3-min wash-in/5-min washout pr...

Sorting of cargoes in endosomes occurs through their selective enrichment into sorting platforms, where transport intermediates are generated. The WASH complex, which directly binds to lipids, activates the Arp2/3 complex and hence actin polymerization onto such sorting platforms. Here, we analyzed the role of actin polymerization in the physiology of endosomal domains containing WASH using qua...

Undernutrition is the underlying cause of 45 percent of child deaths each year.1 The term undernutrition covers three primary anthropometric measures: stunting, which is low height for age; wasting, which is low weight for height; and underweight, which is low weight for age. Despite targeted and comprehensive nutrition-specific interventions, the persistent presence of undernutrition globally ...