It is difficult to assess the transport pathways that carry low-density lipoprotein (LDL) into the artery wall in vivo, and there has been no previous in vitro study that has examined transendothelial transport under physiologically relevant pressurized (convective) conditions. Therefore, we measured water, albumin, and LDL fluxes across bovine aortic endothelial cell (BAEC) monolayers in vitro...

This study was undertaken to investigate the effects of three media, volume and type of oil and frequency of observation on the in vitro development of mouse zygotes. B6CBF1 female mice (4 to 6 wk old) were superovulated using PMSG/hCG and mated with a proven fertile male of the same strain. Putative zygotes with polar bodies were collected from the oviducts of mated mice, 25-28 h after hCG inj...

Bifidobacteria colonize the gut of various mammals, including humans, where they may metabolize complex, diet-, and host-derived carbohydrates. The glycan-associated metabolic features encoded by bifidobacteria are believed to be strongly influenced by cross-feeding activities due to the co-existence of strains with different glycan-degrading properties. In this study, we observed an enhanced g...

Essential oils are volatile compounds, extracted from plants, which have a strong odor. These compounds are known for their antibacterial and antifungal properties. However, data concerning degradation of mycotoxins by these metabolites are very limited. The aim of the present study was to investigate the effect of essential oils (cedarwood, cinnamon leaf, cinnamon bark, white grapefruit, pink ...

In this work in vitro culture conditions for three species belonging to the Labiatae family were studied. In particular, a micropropagation protocol of Lavandino cv Grosso (Lavandula officinalis Chaix × Lavandula latifolia Villars) was developed. As regards this species it was verified that the hyperhydricity symptoms of axillary shoots were related to the agar concentration in the culture medi...

Apoptosis was monitored in polymorphonuclear leukocytes (PMNs) cultured under mildly acidic, neutral, and alkaline conditions. Within 3 h, 9.0% of the PMNs underwent apoptosis at pH 6.7, as did 12% at pH 7.2, 38% at pH 7.7, and 60% at pH 8.2. Inhibitors of serine proteases, caspase-1, or caspase-3 significantly inhibited PMN apoptosis at pH 8.2, suggesting an involvement by these enzymes.

Sir, WethankDrsClark andLaskin for their interest in our recentlypublished systematic review of the effects of antiphospholipid antibodies on cultured placental cells in vitro.We agreewithmany of the authors’ comments and their suggestions for future investigations. However, in suggesting that trophoblast plugs prevent antiphospholipid antibodies accessing the placenta in early gestation, we be...

Significance to Industry: Magnolia (M. liliiflora ‘Nigra’× M. stellata ‘Rosea’) ‘Ann’ (NA 28344; PI 326570) is a member of the ‘Little Girl’ series of magnolias that have become widely popular. Magnolia ‘Ann’ is characterized by a desirable combination of traits including prolific and remontant flowering and a shrubby form. Due to difficulties in propagating magnolias from cuttings or grafting,...

objective: intravascular catheter-associated infection has been increasing hospitalization in post-surgery patients mainly due to microbial colonization of the catheter surface and formation of a superficial biofilm layer. the present study is aimed in developing an effective antibacterial device which can prevent colonization of organisms by modification of catheter. methods: in the present st...