PURPOSE To investigate the vitreous findings in patients with cicatricial retinopathy of prematurity (ROP) who underwent retinal cryopexy and/or photocoagulation during the acute phase of the disease. METHODS Vitreous findings were evaluated in 15 patients (29 eyes) with cicatricial ROP by slit-lamp biomicroscopy and indirect ophthalmoscopy. RESULTS The ocular examination revealed that all ...

PURPOSE Changes in gene expression were investigated after treatment of cultured human retinal pigment epithelial (RPE) cells with vitreous. This may have implications for proliferative diseases such as proliferative vitreoretinopathy. METHODS Cells were cultured in the presence or absence of human vitreous, and gene expression was examined using the differential display polymerase chain reac...

PURPOSE To develop an automated method for quantifying vitreous signal intensity on optical coherence tomography (OCT), with particular application for use in the assessment of vitreous inflammation. METHODS This retrospective, observational case-control series comprised 30 patients (30 eyes), with vitreous haze secondary to intermediate, posterior, or panuveitis; 12 patients (12 eyes) with u...

PURPOSE To investigate the vitreous and plasma levels of vascular endothelial growth factor (VEGF) in patients with proliferative diabetic retinopathy (PDR) and to determine whether they predict a disease prognosis after primary vitrectomy. METHODS Fifty patients (50 eyes) with PDR who underwent pars plana vitrectomy (PPV) and 56 healthy controls (56 eyes) were enrolled in this retrospective ...

For the first time, vitreous oxygen tension in the human vitreous body was measured using a polarographic oxygen electrode during vitreous surgery. The same method was used in a rabbit experimental model. In the human eye, the mean oxygen tensions of the anterior peripheral vitreous body, central vitreous body and posterior vitreous body were 16.7 +/- 3.7 mm Hg (mean +/- 1 SD), 15.9 +/- 2.8 mmH...

Background: Anterior vitreous incarceration is a condition in which vitreous prolapses into the anterior chamber and passes through a microscopic wound at an incisional site. The condition can be identified as a vitreous strand leading to the wound site during slit lamp examination. If the vitreous strand penetrates through all the corneal layers on to the extraocular surface it becomes vitreou...

PURPOSE To evaluate the influence of sclerotomy use during vitrectomy (vitreous cutter, illumination probe, or infusion-line entrance) on postoperative vitreous incarceration using an experimental model of vitrectomized eye. MATERIALS AND METHODS Experimental, randomized, and observer-masked study in which 23-gauge transconjunctival sutureless vitrectomy was performed in cadaveric pig eyes. P...

PURPOSE Aging of the vitreous body can result in sight-threatening pathology. One aspect of vitreous aging is liquefaction, which results from the vanishing of collagen fibrils. We investigated the possibility that trypsins are involved in vitreous type II collagen degradation. METHODS Immunohistochemistry and western blotting were used for detecting and locating trypsin isoforms in the vitre...

While the mouse retina has emerged as an important genetic model for inherited retinal disease, the mouse vitreous remains to be explored. The vitreous is a highly aqueous extracellular matrix overlying the retina where intraocular as well as extraocular proteins accumulate during disease.(1-3) Abnormal interactions between vitreous and retina underlie several diseases such as retinal detachmen...

PURPOSE The goals of this study were to evaluate the safety of office-based vitreous sampling, and determine the utility of these samples with multiplex cytokine analysis. METHODS Vitreous samples were collected from office-based needle aspiration and the rate of adverse events during follow-up was reviewed. The vitreous cytokine concentrations in a subset of patients with diabetic macular ed...