Serum samples from 51 apparently healthy breeding bulls were screened for bovine herpesvirus-1 (BHV-1) antibodies using an avidin-biotin enzyme-linked immunosorbent assay, revealing a sero-positive prevalence rate of 45.09%. Semen samples were then collected from 12 of the sero-positive and 12 of the sero-negative bulls and tested for BHV-1 antigen using both a virus isolation assay and a polym...

THE first convincing isolation of the trachoma virus was made by T'ang, Chang, Huang, and Wang (1957) using embryonate eggs: their findings were confirmed by Collier and Sowa (1958), who also showed that clinical trachoma followed inoculation of a human volunteer with isolated virus. Isolation of the virus from patients with trachoma in Australia has not previously been reported although the di...

THE first convincing isolation of the trachoma virus was made by T'ang, Chang, Huang, and Wang (1957) using embryonate eggs: their findings were confirmed by Collier and Sowa (1958), who also showed that clinical trachoma followed inoculation of a human volunteer with isolated virus. Isolation of the virus from patients with trachoma in Australia has not previously been reported although the di...

Duck beak atrophy and dwarfism syndrome (BADS) is a newly emerged disease in China since 2015. In October 2017, an unidentified disease occurred in Cherry Valley ducks, Chongqing municipality, the southwest of China. The affected birds showed short beak and growth retardation clinical signs. The disease caused approximately 20.00% morbidity and serious weight loss due to retarded growth. In ord...

NCI-H292 mucoepidermoid carcinoma cells from human lungs were shown in an earlier report to be a fully adequate substitute for primary rhesus monkey kidney (MK) cells for the isolation and propagation of the human paramyxoviruses. Although sensitivity for ortho- and paramyxoviruses was the principal reason for using MK cells, the cells were also sensitive to many other viruses, which constitute...

Background and Aims: A multiplex transcription-polymerase chain reaction (m-PCR) was developed for direct detection and discrimination between canarypox virus (CPV) and other avian poxvirus (APV). Materials and Methods: Three compatible primer sets were designed for m-PCR amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved APV genes. Results: Results showed th...

Dengue virus isolation and serotyping was carried out in 105 clinically-suspected cases of dengue fever by the mosquito inoculation and cell culture techniques to find a suitable virus isolation technique for a resource-constrained country like Bangladesh and to identify the serotypes responsible for the dengue outbreak in 2000. Virus was identified by direct fluorescent antibody technique (DFA...

Testing for the presence of hepatitis C virus (HCV) in blood derivatives used in clinical medicine is of enormous importance to ensure the production of safe preparations. So far, no system has been developed for the isolation and detection of hepatitis C virus from blood derivatives. The recently introduced commercial kit for the detection of hepatitis C virus is designed for the isolation and...