background: previous studies have shown that morphine consumption during pregnancy may cause delay or defect of embryo development or abnormal nervous system function in the human and animal models. in the present study, the highest density of morphine accumulation in the central nervous system of rat embryos was evaluated using c14‑morphine. methods: female wistar rats (w 170‑200 g) used and w...

developing a culture system for preantral follicles has important biotechnological implications due to the potential to produce a large number of oocytes for embryo production and transfer. to accomplish this goal, the present study was aimed to culture preantral follicles in the presence of different media, sera and fsh concentrations. six-week-old preantral follicles (95 ± 5 µm) were cultured...

Drug delivery systems comprising vesicles prepared from one amphiphile encapsulating vesicles prepared from a second amphiphile have not been prepared previously due to a tendency of the bilayer components of the different vesicles to mix during preparation. Recently we have developed polymeric vesicles using the new polymer-palmitoyl glycol chitosan and cholesterol in a 2:1 weight ratio. These...

We develop a three-dimensional immersed boundary chromodynamic multicomponent lattice Boltzmann method capable of simulating vesicles, such as erythrocytes. The presented is encapsulated in single framework, where the application force automatically adaptive interfacial region results correct vesicle behavior. also set down methodology for computing principal curvatures surface three-dimensiona...

At an initial step during synaptic vesicle recycling, dynamin and adaptor proteins mediate the endocytosis of synaptic vesicle components from the plasma membrane. StonedA and stonedB, novel synaptic proteins encoded by a single Drosophila gene, have predicted structural similarities to adaptors and other proteins implicated in endocytosis. Here, we test possible roles of the stoned proteins in...

objectives: it was aimed to investigate the effects of different doses of follicle stimulating hormone (fsh) and activin a on the growth and maturation of preantral mouse follicles during the in vitro culture. methods: preantral follicles (90-100 µm in diameter) were harvested from 6-8 week-old syrian mice and cultured in tcm199 culture medium for 6 days to see the effect of fsh and activin a. ...

The administration of hydrophobic actives and drugs for medical or cosmetical purposes generally requires a formulation that ensures adequate water solubility, which can be achieved through the encapsulation in liposomes. For vehiculation (+)-usnic acid (UA), compound with antioxidant activity, we have prepared liposomes one-step process using compressed CO2. investigated formulations are mainl...

Mesoscale simulations are performed to study the complexes between a dendrimer and a vesicle of amphiphilic molecules. In particular, the assembled structures and dynamics of these complexes are investigated by tuning vesicle size and the surface tension of vesicle membrane. Our simulations demonstrate that a dendrimer-based bulge containing amphiphilic molecules forms in the vesicle membrane w...

Vesicle sharing between synaptic boutons is an important component of the recycling process that synapses employ to maintain vesicle pools. However, the mechanisms supporting and regulating vesicle transport during the inter-synaptic exchange remain poorly understood. Using nanometer-resolution tracking of individual synaptic vesicles and advanced computational algorithms, we find that long-dis...

DNA origami nanopore with large size of a 10 nm square, equipping tunable lid, enables size-selective molecular transportation through the lipid membrane giant vesicle.