The subcellular distribution of free amino acids was examined in 0.25 M sucrose homogenates of rat livers cycbtally perfused for 60 min with an unsupplemented (control) medium. An unexpectedly high (30%) proportion of total intracellular valine was found in the mitochondrial-lysosoma1 fraction; 57% was in the 100,000 x .o supernatant; and 9% and 4% were in the nuclear and microsomal fractions, ...

The formation constants (log K), of the complexes formed between a number of amino acids(glycine , L-valine and L-alanine) with p-sulfonatocalix [4] arene at varying temperatures (25± 0.1to 65 ± 0.1 °C) in aqueous solutions and at natural pH of p-sulphonato-calix [4] arene (pH=3.2) bymeans of UV-Vis spectrophotometeric technique have been investigated. At this pH the guestmolecule is in its cat...

The activities of six enzymes which take part in the oxidation of valine by Pseudomonas putida were measured under various conditions of growth. The formation of four of the six enzymes was induced by growth on d- or l-valine: d-amino acid dehydrogenase, branched-chain keto acid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase, and methylmalonate semialdehyde dehydrogenase. Branched-chain amin...

Unfertilized and fertilized eggs of the sea urchin Lytechinus pictus were preloaded with [C]valine and exposed to individual solutions of each of the twenty 'coded' [C]amino acids in artificial sea water. After 1 h incubation the amount of radioactivity in the medium was determined. The radioactivity was effectively displaced by most of the other neutral [C]amino acids that are known to compete...

This study was conducted to examine the effects of dietary valine on the physical and flavor characteristics, fatty acid (FA) profile, antioxidant status and Nrf2-dependent antioxidant enzyme gene expression in the muscle of young grass carp (Ctenopharyngodon idella) fed increasing levels of valine (4.3, 8.0, 10.6, 13.1, 16.9 and 19.1 g/kg) for 8 weeks. Compared with the control group, the grou...

Regulation of the biosynthesis of four of the five enzymes of the isoleucine-valine pathway was studied in Saccharomyces cerevisiae. A method is described for limiting the growth of a leucine auxotroph by using valine as a competitor for the permease. Limitation for isoleucine and valine was accomplished by the use of peptides containing these amino acids conjugated with glycine as nutritional ...

Streptomyces antibioticus synthesizes a mixture of actinomycins (I to V), each of which possesses 2 moles of n-valine per mole of antibiotic (1, 2) ; occasionally a sixth substance is produced in trace amounts. Streptomyces chrysomallus, on the other hand, forms a mixture consisting of actinomycins IV (containing 2 moles of n-valine), VI (containing 1 mole of n-valine and 1 mole of n-alloisoleu...

The mode of metal ion and water binding to the amino acid valine is investigated using both theory and experiment. Computations indicate that without water, the structure of valine is nonzwitterionic. Both Li(+) and Na(+) are coordinated to the nitrogen and carbonyl oxygen (NO coordination), whereas K(+) coordinates to both oxygens (OO coordination) of nonzwitterionic valine. The addition of a ...