Replication of staphylococcal multiresistance plasmid pSK41 is negatively regulated by the antisense transcript RNAI. pSK41 minireplicons bearing rnaI promoter (PrnaI) mutations exhibited dramatic increases in copy number, approximately 40-fold higher than the copy number for the wild-type replicon. The effects of RNAI mutations on expression of the replication initiator protein (Rep) were eval...

The replication-associated (Rep) protein of porcine circovirus (PCV) was suggested to play an essential role in the replication and translation of viral DNA. In this study, one monoclonal antibody (mAb) specific for Rep protein of porcine circovirus type 1 (PCV1), two mAbs against Rep protein of porcine circovirus type 2 (PCV2), and five mAbs to both Rep protein of PCV1 and PCV2 were generated ...

The effects of recombinant human erythropoietin (rEp) on murine hematopoietic progenitors were studied using a serum-free culture. A high concentration of rEp stimulated the formation of mixed erythroid-megakaryocyte colonies (EM colonies) and blast cell colonies, as well as erythroid colonies, erythroid bursts, and megakaryocyte colonies from normal mouse bone marrow cells. Direct effects of r...

introduction: p. aeruginosa is an important multi-drug resistant (mdr) opportunistic pathogen in hospital-acquired infections. several methods based on dna analysis have been used for investigating genetic diversity of p. aeruginosa isolates. among them, rep‑pcr typing has been introduced as a rapid, low cost method with high discriminatory power. the aims of this study were to investigate geno...

The large Rep proteins, p68 and p78, function as master controllers of the adeno-associated virus type 2 (AAV2) life cycle, involved in transcriptional control, in latency, in rescue, and in viral DNA replication. The p5 promoter may be the nucleic acid complement to the large Rep proteins. It drives expression of the large Rep proteins, it undergoes autoregulation by Rep, it undergoes inductio...

The long wavelength edge of the major chlorophyll absorption feature in the spectrum of a vegetation canopy moves to longer wavelengths with an increase in chlorophyll content. The position of this red-edge has been used successfully to estimate, by remote sensing, the chlorophyll content of vegetation canopies. Techniques used to estimate this red-edge position (REP) have been designed for use...

The CMT1A-REP repeat on chromosome 17p11.2-12 is proposed to mediate misalignment and meiotic unequal crossover leading to a 1.5 Mb pair duplication associated with Charcot-Marie-Tooth neuropathy type 1A (CMT1A) and a reciprocal deletion associated with hereditary neuropathy with liability to pressure palsies (HNPP). Restriction enzyme endonuclease mapping indicated that the size of the CMT1A-R...

While almost all collision detection algorithms have been linked to a particular object representation scheme, few attempts have been made using hybrid methods to exploit the advantages of multiple representations of objects. In this paper we present a collision detection algorithm based on a dual b-rep/CSG representation of concave polyhedra initially represented in b-rep. Our method naturally...

The actual condition of vegetation cover is represented by its spectral signature. Photosynthetic active vegetation is characterized by a low reflectance in the red and a high reflectance in the near infrared spectra. This specific feature is used to calculate different vegetation indices. One index is the MERIS Global Vegetation Index (MGVI), the other one is the Red Edge Position (REP), which...

The majority of cases of Charcot-Marie-Tooth type 1A (CMT1A) and of hereditary neuropathy with a liability to pressure palsies (HNPP) are the result of heterozygosity for the duplication or deletion of peripheral myelin protein 22 gene (PMP22) on 17p11.2. Southern blots, pulsed-field gel electrophoresis (PFGE), fluorescence in situ hybridization (FISH) and polymorphic marker analysis are curren...