The model rumen Firmicutes organism Ruminococcus albus 8 was grown using ammonia, urea, or peptides as the sole nitrogen source; growth was not observed with amino acids as the sole nitrogen source. Growth of R. albus 8 on ammonia and urea showed the same growth rate (0.08 h(-1)) and similar maximum cell densities (for ammonia, the optical density at 600 nm [OD600] was 1.01; and for urea, the O...

objective: helicobacter pylori is to blame for one of the most common chronic infections in humans and can cause peptic ulcer, gastritis, duodenitis and non-ulcer dyspepsia. developing a reliable test to detect the infection is of great importance. for a variety of reasons, the existing tests including the stool culture, biopsy, pcr, and urea breath test are not up to par. the pcr-elisa test is...

BACKGROUND Helicobacter pylori infection is etiologically associated with some important health problems such as gastric cancer. Because of the high clinical importance of H. pylori infection, development of a noninvasive test for the detection of H. pylori is desirable. METHODS In this study, a loop-mediated isothermal amplification (LAMP) targeted ureC of H. pylori was evaluated on 100 stoo...

Natto is a traditional Japanese food made from soybeans fermented by strains of Bacillus subtilis natto. It gives off a strong ammonia smell during secondary fermentation, and the biochemical basis for this ammonia production was investigated in this study. When natto was fermented by strain r22, ammonia production was shown to involve degradation of soybean proteins releasing amino acids, and ...

Helicobacter pylori urease, a nickel-requiring metalloenzyme, hydrolyzes urea to NH3 and CO2. We sought to identify H. pylori genes that modulate urease activity by constructing pHP8080, a plasmid which encodes both H. pylori urease and the NixA nickel transporter. Escherichia coli SE5000 and DH5alpha transformed with pHP8080 resulted in a high-level urease producer and a low-level urease produ...

BACKGROUND Helicobacter pylori has recently been detected in the stomach and trachea of cases of sudden infant death syndrome (SIDS) and proposed as a cause of SIDS. AIMS To establish the incidence of H pylori in the stomach, trachea, and lung of cases of SIDS and controls. METHODS Stomach, trachea, and lung tissues from 32 cases of SIDS and eight control cases were examined retrospectively...

Helicobacter pylori is the causative agent of chronic gastritis and peptic ulcer diseases and is also a risk factor for gastric cancer. Since culture of Helicobacter pylori is relatively insensitive and cumbersome, PCR-based molecular detection and typing of this organism is gaining importance for strain differentiation. The aim of present study was to apply molecular methods for detection and ...

BACKGROUND The current study aimed to detect the presence of Helicobacter pylori in some water systems in Egypt as well as in blood samples of patients suffering from stomach ulcers. METHODS Fifty-one water samples collected from some Egyptian governorates; Giza, Alexandria, Monofia, Beheira, Minya, Sohag, was subjected to physical and chemical analysis. Urease gene ureC, a highly specific se...