There is a long and distinguished history of research on communication and other types of social networks. Some trace the intellectual foundations of this tradition to the work of John Stuart Mills and Herbert Spencer in the 19th century (Mattelart, 2000/1996), and others to the pioneering empirical work of Jacob Moreno in the early 20th century (Wasserman & Faust, 1994). Much has been learned ...

Next-generation sequencing is a fast and cost-effective way to obtain sequence data for nonmodel organisms for many markers and for many individuals. We describe a protocol through which we obtain orthologous markers for the crested newts (Amphibia: Salamandridae: Triturus), suitable for analysis of interspecific hybridization. We use transcriptome data of a single Triturus species and design 9...

Digital PCR (dPCR) is a highly accurate molecular approach, capable of precise measurements, offering a number of unique opportunities. However, in its current format dPCR can be limited by the amount of sample that can be analysed and consequently additional considerations such as performing multiplex reactions or pre-amplification can be considered. This study investigated the impact of duple...

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

Cassava mosaic disease (CMD) is a major viral adversely affecting cassava production in Africa and Asia. Genomic regions conferring resistance to the have been mapped African germplasm through biparental quantitative trait loci (QTL) mapping genome-wide association studies. To facilitate utilization of these markers breeding pipelines support selections, proof-of-concept technical biological va...

Biomarkers are becoming increasingly more important in clinical decision-making, as well as basic science. Diagnosing myocardial infarction (MI) is largely driven by detecting cardiac-specific proteins in patients' serum or plasma as an indicator of myocardial injury. Having recently shown that cardiac myosin binding protein-C (cMyBP-C) is detectable in the serum after MI, we have proposed it a...