conclusions our results demonstrated that r-fla-a could induce cellular and humoral immune response as proper stimulant of poly-isotypic humoral responses. results immunized mice with adjuvanted flagellin showed a considerably increased lymphocyte proliferation compared with the control group (p = 0.004). high level of il-4 and ifn-γ secretion was observed in immunized mice compared with the co...

background: patients with primary sjögren’s syndrome (pss) produce functional igg against cholinoreceptor of exocrine glands modifying their activity. the aim of the present work was to demonstrate pss igg antibodies (pss igg) interacting with m 3 muscarinic acetylcholine receptors (machr) of rats submandibular glands that alter mucin release and production via phospholipase c (plc) and cycloox...

background and aims: primary infections of rubella and human cytomegalovirus (hcmv) can lead to severe complications in pregnancy. the screening of rubella and hcmv in pregnant women is not routinely carried out in iran. this cross sectional study aimed to investigate the seroprevalence of hcmv and rubella infections in neonates with and without congenital defects. materials and methods: this s...

objectives the current study aimed to assess relationship between s/co rates and hcv-rna levels in the laboratory to identify hcv viremia in patients with a positive anti-hcv. patients and methods all serum samples were assayed for anti-hcv by elisa method. a total of 265 anti-hcv positive patients were tested for hcv-rna testing by quantitative method using artus hcv rg real-time polymerase ch...

conclusions gp55 of human cmv is considered as an important candidate for immunotherapy. in this study, we selected three specific clones against gp55. the scfvs reacted only with the corresponding peptide in a positive elisa. the scfv2 with 68.8% neutralizing effect showed the potential to be considered for prophylaxis and treatment of cmv infections, especially in solid organ transplant recip...

background: inhibitory antibody to exogenous factor viii (fviii) is a major complication of hemophilia treatment. this study was conducted to determine the prevalence of inhibitor antibody directed against fviii.methods: from may 2010 to may 2011, 52 patients with severe hemophilia a admitted in amirkola children’s hospital were evaluated. those who had abnormal mixing study, antibody against f...

background: nanoparticles have been considered as promising tools because of their high applicability. recently, nanoparticles have been evaluated for their ability to increase the immune responses as adjuvants. silver-nanoparticles (agnps) have shown promising results in enhancing th-2 immune responses and to produce potent neutralizing antibodies. neutralizing antibodies are considered as the...

background: antinuclear antibodies (anas) in women with recurrent miscarriage have been reported. the presence of moderate to high titers of these antibodies represents an autoimmune condition that can endanger the health of the fetus in pregnant women. objective: in this study, we evaluated the prevalence of anas in iranian women with a history of two or more unexplained abortion. materials an...

Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

the culture media optimization is an inevitable part of upstream process development in therapeutic monoclonal antibodies (mabs) production. the quality by design (qbd) approach defines the assured quality of the final product through the development stage. an important step in qbd is determination of the main quality attributes. during the media optimization, some of the main quality attribute...