BACKGROUND Zona pellucida opening for blastomere removal can be done by mechanical or chemical means, or by laser. So far, only limited data on the use of laser systems for zona drilling in cases of PGD are available. METHODS Results of embryo biopsy and outcome of PGD in two periods were compared. In the first period, acid Tyrode medium was used for zona drilling. In the second period, zona ...

The effects of lyotropic (swelling) anions (Cl(-), Br(-), NO(3)(-) and I(-)) on contractile properties of fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (SOL) muscles were investigated in vitro at 20 degrees C and 35 degrees C. Isolated muscles bathed in anionic Tyrode solution were stimulated directly and isometric single twitches and fused tetanic contractions were recorde...

IT MAY BE CONCLUDED THAT, UNDER THE CONDITIONS OF THE EXPERIMENTS: 1. The duration of life of fibroblasts is not altered by the presence of 7 per cent serum in a medium composed of fibrin and Tyrode solution, but is slightly decreased when the concentration of the serum reaches 25 per cent. 2. Fibroblasts cultivated in serum or in Tyrode solution are only in a condition of survival; they do not...

Objective: This study was carried out to examine the effect of functional modification of Caactivated Cl currents on contractile activities of isolated gravid rat myometrium. Materials and Methods: Spontaneous rhythmic and oxytocin-induced contractile activities of gravid rat myometrium in the presence and absence of Cl (in the bathing medium) were studied in a tissue bath system. The effects o...

OBJECTIVE To investigate the action of N-acetylcysteine (NAC) on rat isolated ileal contractility, and to determine the effects of NAC on histopathological changes on ileal tissue. METHODS The study took place at the Faculty of Medicine, Ankara University, Ankara, Turkey, in January 2003. Adult Wistar rats were used in all experiments. Two groups were designed. The experimental group, to whic...

Onion (Allium cepa) bulb from Liliaceae has antioxidant, spasmolytic and antihypertensive activities. The aim of present study was to investigate the antispasmodic effect of onion peel on rat ileum contractility. Onion peel powder was extracted by maceration in 70% alcohol for 72 h. A terminal portion of ileum from male Wistar rat was dissected and its contractions were recorded isotonically in...

IT has already been shown (1933, 1936) that chick fibroblasts (mesenchyme cells derived from the heart or from periosteal tissue) can be reduced to a state in which growth by cell division is almost at a standstill and that, by the addition to the medium of embryo tissue juice, the cells can be caused to divide again. The higher the concentration of embryo juice used, the greater is the number ...

Lidocaine depresses automaticity in cardiac Purkinje fibers by decreasing the slope of slow diastolic depolarization, but the mechanisms of this effect are poorly understood. To test the proposal that the antiautomatic effect of lidocaine might be mediated by an increase in membrane potassium conductance, transmembrane voltage (V(m)) was measured in Purkinje fibers perfused with sodium-deficien...

1. Vascular reactivity and noradrenaline overflow were studied in Tyrode solution-perfused kidneys from young (6 weeks) normotensive and spontaneously hypertensive (SH) rats. 2. The vasoconstrictor response to nerve stimulation was greater in the kidneys from young SH rats than in those from young control rats. 3. Nerve stimulation evoked a greater release of noradrenaline in the kidneys from y...

The effect of different frequencies of direct subtetanic electrical stimulation (14, 20, and 30 Hz) and aminophylline (AMPh) pretreatment on the effect of N(G)-nitro-L-arginine methyl ester (L-NAME) (1 mM) on isolated rat diaphragm was investigated. L-NAME potentiated tension developed (Td) in the diaphragm pretreated with a single concentration of AMPh (1.08 mM) in a frequency-dependent manner...