Successful targeting methods represent a major hurdle to the use of retroviral vectors in cell-specific gene-delivery applications. We recently described an approach for retroviral targeting with a retroviral receptor-ligand bridge protein that was bound to the cognate cell-surface ligand receptors before viral challenge. We now report a significant improvement made to this viral targeting meth...

The transcription factor Blimp-1 has emerged as a regulator of cell fate in embryonic (germ cell) and adult (B- and T-cell immune effector and epithelial) lineages. It has also been proposed to act as a tumor suppressor in B-cell malignancy. Here, we present a novel in vivo system enabling the targeted genetic manipulation of cells expressing Prdm1, the gene encoding Blimp-1. We created bacteri...

The effect of formalin on the organoleptic parameters, biochemical composition and storage quality of iced and frozen stored giant freshwater prawns (Macrobrachium rosenbergii) was investigated. The organoleptic characters such as appearance, textural condition, colour, odour of both formalin added and non-added prawns were evaluated. The quality of non-added prawns was judged good up to first ...

We consider a family of high-order, weighted essentially non-oscillatory central schemes (CWENO) for approximating solutions of one-dimensional hyperbolic systems of conservation laws. We are interested in the behavior of the total variation (TV) of the approximate solution obtained with these methods. Our numerical results suggest that even though CWENO methods are not total variation diminish...

The fresh mackerel was frozen using four different quick-freezing methods, such as refrigerator freezing, quick freezer spiral tunnel freezing and plate freezing. Water retention, color, texture, electrical conductivity, total volatile base nitrogen (TVB-N), K value, malondialdehyde (MDA), sulfhydryl group protein secondary structure were used physicochemical indicators to evaluate the changes ...

Total Volatile Base Nitrogen (TVB-N) and Trimethylamine (TMA-N) contents of Vetki (Lates calcarifer) were investigated following Conway’s Microdiffusion technique at 4 hour interval in Fish Quality Control Laboratory, Fisheries Marine Resource Technology Discipline, Khulna University. Observed TVB-N TMA-N value did not vary significantly among different size classes. At fresh condition, content...

BACKGROUND: Freezing is a common way and one of the best methods of seafood preservation for long periods of time; however, the freeze thawing process may influence the quality of food. OBJECTIVES: Oxidation and denaturation of proteins, sublimation and recrystallization of ice crystals can cause changes in the quality of the frozen products. This study was aimed to evaluate the effect of three...

The determination of total volatile bases (TVB) is probably the oldest objective chemical method for assessing the spoilage of fishery pro­ ducts. It was proposed about a century ago and was already published as standard method for the inspection of fish in Germany in the first years of this century (KÖNIG, 1910). This simple method is still widely used. A European Union directive on fish hygie...

We have seen important strides in our understanding of mechanisms underlying stroke recovery, yet effective translational links between basic and applied sciences, as well as from big data to individualized therapies, are needed to truly develop a cure for stroke. We present such an approach using The Virtual Brain (TVB), a neuroinformatics platform that uses empirical neuroimaging data to crea...

The quality and nutritional value for both fish protein concentrate (FPC) made from tilapia by-products as well as salt-biscuits supplemented with 5% FPC were evaluated. The resultant product (FPC) was composed (on wet weight) of 4.18% moisture, 68.83% protein content, 0.75% lipid and 25.39% ash and it was more uniform in color and texture. In addition, the pH value was 6.67 and total volatile ...