AIMS To determine sperm nuclear DNA integrity and to investigate the relation between fenvalerate (FE) exposure and spermatozoa DNA damage. METHODS Sperm DNA fragmentation was detected by a modified alkaline single cell gel electrophoresis (Comet) assay and a terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) assay. The olive tail moment (OTM) and percentage tail D...

BACKGROUND Sperm DNA integrity (SDI) is an important factor in the prognosis of male fertility. Here we compare the toluidine blue (TB) image cytometry test, recently proposed by us for SDI assessment, with two other tests-the sperm chromatin structure assay (SCSA) and the terminal nick-end labelling (TUNEL) assay. METHODS Sperm samples from 35 men were evaluated for standard sperm parameters...

We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses...

BACKGROUND The presence of apoptotic myocytes has been reported in human hearts with dilated cardiomyopathy (DCM) on the basis of a positive finding of DNA in situ nick end-labeling (TUNEL). However, ultrastructural evidence of myocyte apoptosis has not been obtained. METHODS AND RESULTS A total of 80 endomyocardial biopsies were obtained from right and left ventricles of 20 patients with DCM...

guish between apoptotic and necrotic cells, we performed an Annexin V assay with propidium iodide exclusion. In accordance with our TUNEL assay, .95% of the cells were Annexin V positive (unpublished results). Less than 5% of these Annexin V-positive cells showed simultaneous incorporation of propidium iodide. We therefore conclude that .95% of the plasmaderived cells are indeed in apoptosis an...

The apoptotic effect of bacteria-derived beta-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. beta-Glucan of 10, 50, and 100 microg/mL decreased...

Although pyrimethamine (Tindurin™) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. We design this study to evaluate its anti-tumoral effect on a model of cell line. Methods: The cytotoxic influence of Pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. The po...

Many studies have now confirmed that sperm DNA fragmentation (SDF) is associated with a poorer outcome of some forms of assisted reproduction technology. For this reason, SDF is an important parameter to evaluate in male fertility assessment. TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay coupled to flow cytometry is one of the most promising methods for SDF quantifi...

although pyrimethamine (tindurin™) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. we design this study to evaluate its anti-tumoral effect on a model of cell line. methods: the cytotoxic influence of pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. the po...