Background: Bax and Bcl-2 are the major members of Bcl-2 family whose play a key role in tumor progression or inhibition of intrinsic apoptotic pathway triggered by mitochondrial dysfunction. Therefore, the balance between pro- and anti-apoptotic members of this family can determine the cellular fate. Methods: In this study, the relative level of mRNA expression of Bax and Bcl-2 genes was deter...

A growing area of research is focused on cancer therapy, and new therapeutic approaches are welcomed. Mesenchymal stem cell (MSC)-based gene therapy is a promising strategy in oncology. Intrinsic tropism and migration to tumor microenvironment with off lights are attractive features of this type of cell carrier. In this way, suicide genes have also found a good platform for better performance a...

Abstract TIGIT is an immune checkpoint inhibitor expressed by effector CD4+ and CD8+ T cells, NK regulatory cells (Tregs). Inhibition of TIGIT-ligand binding using antagonistic anti-TIGIT mAbs has shown in vitro potential to restore T-cell function therapeutic efficacy murine tumor models when combined with anti–PD(L)-1 antibody. In the current work, we demonstrate broader expression than previ...

Palmitate, the enzymatic product of FASN, and palmitate-derived lipids support cell metabolism, membrane architecture, protein localization, and intracellular signaling. Tubulins are among many proteins that are modified post-translationally by acylation with palmitate. We show that FASN inhibition with TVB-3166 or TVB-3664 significantly reduces tubulin palmitoylation and mRNA expression. Disru...

Abstract Glioblastoma are incurable primary tumors of the central nervous system. Novel treatment options urgently needed. Recently, we investigated molecular effects disrupting (b)HLH network, a frequently altered and tumor-promoting transcriptional network in glioma, by introducing dominant-negative variant E47 protein. The identified downstream anti-tumor included DNA damage response pathway...

Approved angiogenesis inhibitors generally target certain growth factors or their receptors, which exist in both normal and cancerous cells; this results suppression of cell-signaling pathways throughout the body causes adverse effects. For reason, there is a need for new inhibitor that can specifically tumor vasculature. In our previous study, we revealed doppel, prion-like protein, was overex...

introduction: transient cavitations induced by low frequency irradiation of ultrasound can be used to treat tumors. previous studies in in-vitro experiments have shown that induced cavitation by dual or multiple frequencies of ultrasound is greater than induced cavitation by single frequency irradiation. in this study, we compared and evaluated the treatment effects of dual frequency irradiatio...

Background: Human epidermal growth factor receptor 2 (HER2) has a crucial role in several malignancies. The extracellular domain of HER2 (HER2-ECD) has been extensively employed as an important target in passive and active immunotherapy. Isolated recombinant prokaryotic HER2-ECD subdomains were previously found to be ineffective in inducing anti-tumor antibody response. Objective: To employ rec...