three tuberculous, twenty-one non-tuberculous mycobacterial (ntm) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of dna fragments in a polymerase chain reaction with single universal primer (up-pcr). a rather wide limit of tolerance for variations in procedure of pcr mixture preparation and thermocycling parameters was found. there...

BACKGROUND Non-tuberculous mycobacteria have long been identified as capable of causing human disease and the number at risk, due to immune-suppression, is rising. Several reports have suggested incidence to be increasing, yet routine surveillance-based evidence is lacking. We investigated recent trends in, and the epidemiology of, non-tuberculous mycobacterial infections in England, Wales and ...

OBJECTIVE To assess risk factors and mycobacterial agents in mycobacterial adenitis. DESIGN Cross sectional involving comparison analysis of high-risk groups. SETTING Seven hospitals in rural and semi-rural districts of Arusha. SUBJECTS The study comprised of 457 patients of clinically diagnosed mycobacterial adenitis. INTERVENTIONS Biopsy materials were cultured and identification of m...

Bovine tuberculosis was last detected in cattle and pigs in the Czech Republic in 1995. Since March, 31, 2004 (Commission Decision No. 2004/320/EC) the Czech Republic has been included amongst states free from bovine tuberculosis within the European Union. The purpose of the present study was to evaluate results of intravital and post-mortem diagnosis of mycobacterial infections in slaughtered ...