نتایج جستجو برای: tryparedoxin peroxidase

تعداد نتایج: 30182  

Journal: :Journal of vector borne diseases 2012
Sharifeh Khosravi Saied Hossein Hejazi Mortaza Hashemzadeh Gilda Eslami Hossein Yousofi Darani

BACKGROUND & OBJECTIVES Rapid and accurate diagnosis and identification of Leishmania sp causing cutaneous leishmaniasis is crucial in control and therapeutic programs. The problem of diagnosis with traditional methods is that they have a low sensitivity or time consuming but molecular techniques would be an alternative method for rapid and accurate diagnosis. In this work, tryparedoxine peroxi...

Journal: :The Journal of biological chemistry 2012
Florian Fueller Britta Jehle Kerstin Putzker Joe D Lewis R Luise Krauth-Siegel

In African trypanosomes, the detoxification of broad spectrum hydroperoxides relies on a unique cascade composed of trypanothione (T(SH)(2)), trypanothione reductase, tryparedoxin (Tpx), and nonselenium glutathione peroxidase-type enzymes. All three proteins are essential for Trypanosoma brucei. Here, we subjected the complete system to a high throughput screening approach with nearly 80,000 ch...

2009
Connor Carson Maria Antoniou Maria Begoña Ruiz-Argüello Antonio Alcami Vasiliki Christodoulou Ippokratis Messaritakis Jenefer M. Blackwell Orin Courtenay

Previous studies demonstrated safety, immunogenicity and efficacy of DNA/modified vaccinia virus Ankara (MVA) prime/boost vaccines expressing tryparedoxin peroxidase (TRYP) and Leishmania homologue of the mammalian receptor for activated C kinase (LACK) against Leishmania major challenge in mice, which was consistent with results from TRYP protein/adjuvant combinations in non-human primates. Th...

2014
Calvin A. Henard Eric D. Carlsen Christie Hay Peter E. Kima Lynn Soong

Professional phagocytes generate a myriad of antimicrobial molecules to kill invading microorganisms, of which nitrogen oxides are integral in controlling the obligate intracellular pathogen Leishmania. Although reactive nitrogen species produced by the inducible nitric oxide synthase (iNOS) can promote the clearance of intracellular parasites, some Leishmania species/stages are relatively resi...

2015
Priscila Camillo Teixeira Leonardo Garcia Velasquez Ana Paula Lepique Eloiza de Rezende José Matheus Camargo Bonatto Marcello Andre Barcinski Edecio Cunha-Neto Beatriz Simonsen Stolf

Leishmaniasis is an important disease that affects 12 million people in 88 countries, with 2 million new cases every year. Leishmania amazonensis is an important agent in Brazil, leading to clinical forms varying from localized (LCL) to diffuse cutaneous leishmaniasis (DCL). One interesting issue rarely analyzed is how host immune response affects Leishmania phenotype and virulence. Aiming to s...

Journal: :Clinical and vaccine immunology : CVI 2015
Mariana C Duarte Daniel C Pimenta Daniel Menezes-Souza Rubens D M Magalhães João L C P Diniz Lourena E Costa Miguel A Chávez-Fumagalli Paula S Lage Daniela C Bartholomeu Maria Julia M Alves Ana Paula Fernandes Manuel Soto Carlos A P Tavares Denise U Gonçalves Manoel O C Rocha Eduardo A F Coelho

The serodiagnosis of human tegumentary leishmaniasis (TL) presents some problems, such as the low level of antileishmanial antibodies found in most of the patients, as well as the cross-reactivity in subjects infected by other trypanosomatids. In the present study, an immunoproteomic approach was performed aimed at identification of antigens in total extracts of stationary-phase promastigote an...

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